PUBLICATION
Release of STK24/25 suppression on MEKK3 signaling in endothelial cells confers Cerebral cavernous malformation
- Authors
- Yang, X., Wu, S.T., Gao, R., Wang, R., Wang, Y., Dong, Z., Wang, L., Qi, C., Wang, X., Schmitz, M.L., Liu, R., Han, Z., Wang, L., Zheng, X.
- ID
- ZDB-PUB-230125-4
- Date
- 2023
- Source
- JCI insight 8(5): (Journal)
- Registered Authors
- Keywords
- Angiogenesis, Endothelial cells, Genetic diseases, Stroke, Vascular Biology
- MeSH Terms
-
- Animals
- Endothelial Cells
- Hemangioma, Cavernous, Central Nervous System*/genetics
- Mice
- Phosphorylation
- Protein Processing, Post-Translational
- Protein Serine-Threonine Kinases/genetics
- Zebrafish
- PubMed
- 36692953 Full text @ JCI Insight
Citation
Yang, X., Wu, S.T., Gao, R., Wang, R., Wang, Y., Dong, Z., Wang, L., Qi, C., Wang, X., Schmitz, M.L., Liu, R., Han, Z., Wang, L., Zheng, X. (2023) Release of STK24/25 suppression on MEKK3 signaling in endothelial cells confers Cerebral cavernous malformation. JCI insight. 8(5):.
Abstract
Loss of function mutations in CCM genes and gain of function mutation in the MAP3K3 gene encoding MEKK3 cause cerebral cavernous malformation (CCM). Deficiency of CCM proteins leads to the activation of MEKK3-KLF2/4 signaling, but it is not clear how this occurs. Here we demonstrate that deletion of the CCM3 interacting kinases STK24/25 in endothelial cells cause defects in vascular patterning during development as well as CCM lesion formation during postnatal life. While permanent deletion of STK24/25 in endothelial cells caused developmental defects of the vascular system, inducible postnatal deletion of STK24/25 impaired angiogenesis in the retina and brain. More importantly, deletion of STK24/25 in neonatal mice led to the development of severe CCM lesions. At the molecular level, a hybrid protein consisting of the STK kinase domain and the MEKK3 interacting domain of CCM2 rescued the vascular phenotype caused by the loss of ccm gene function in zebrafish. Our study suggests that CCM2/3 proteins act as adapters to allow recruitment of STK24/25 to limit the constitutive MEKK3 activity that contributes to vessel stability. Loss of STK24/25 causes MEKK3 activation leading to CCM lesion formation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping