PUBLICATION
Dietary Application of the Microalga Lobosphaera incisa P127 Reduces Severity of Intestinal Inflammation, Modulates Gut-Associated Gene Expression and Microbiome in the Zebrafish Model of IBD
- Authors
- Novichkova, E., Nayak, S., Boussiba, S., Gopas, J., Zilberg, D., Khozin-Goldberg, I.
- ID
- ZDB-PUB-230124-1
- Date
- 2023
- Source
- Molecular Nutrition & Food Research 67(6): e2200253 (Journal)
- Registered Authors
- Keywords
- DGLA, Dietary application, IBD, Microalgae, Microbiome
- MeSH Terms
-
- Animals
- Chlorophyta*/genetics
- Chlorophyta*/metabolism
- Diet
- Gastrointestinal Microbiome*
- Gene Expression
- Inflammation
- Inflammatory Bowel Diseases*/drug therapy
- Microalgae*
- Microbiota*
- Zebrafish/genetics
- PubMed
- 36683256 Full text @ Mol. Nutr. Food Res.
Citation
Novichkova, E., Nayak, S., Boussiba, S., Gopas, J., Zilberg, D., Khozin-Goldberg, I. (2023) Dietary Application of the Microalga Lobosphaera incisa P127 Reduces Severity of Intestinal Inflammation, Modulates Gut-Associated Gene Expression and Microbiome in the Zebrafish Model of IBD. Molecular Nutrition & Food Research. 67(6):e2200253.
Abstract
Scope Microalgae are an emerging nutritional resource of biomolecules with potential to alleviate gut inflammation. We explored the anti-inflammatory and immunomodulatory potential of the microalga Lobosphaera incisa P127, which accumulates a rare omega-6 LC-PUFA dihomo-ɣ-linolenic acid (DGLA) under nitrogen starvation. The therapeutic potential of dietary supplementation with P127 was investigated in the zebrafish model of IBD (TNBS-induced colitis).
Methods and results Guts were sampled from zebrafish fed experimental diets for four weeks, before and 24h after TNBS challenge. Diets containing 15% non-starved (Ns) and 7.5 and 15% N-starved (St) algal biomass significantly attenuated the severity of gut injury and goblet cell depletion. In contrast, diets containing 7.5 % Ns and DGLA ethyl ester had no effect on gut condition. Fish fed 15% St, high-DGLA biomass, had the fewest individuals with pathological alterations in the gut. Dietary inclusion of Ns and St distinctly modulated gut-associated expression of the immune and inflammatory genes. Fish fed 15% Ns biomass displayed a coordinated boost in immune gene expression and showed major changes in the gut microbiome prior challenge.
Conclusion Dietary inclusion of L. incisa biomass at two physiological states, ameliorated TNBS-induced gut inflammation, suggesting the synergistic beneficial effects of biomass components not limited to DGLA. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping