PUBLICATION
Myelination generates aberrant ultrastructure that is resolved by microglia
- Authors
- Djannatian, M., Radha, S., Weikert, U., Safaiyan, S., Wrede, C., Deichsel, C., Kislinger, G., Rhomberg, A., Ruhwedel, T., Campbell, D.S., van Ham, T., Schmid, B., Hegermann, J., Möbius, W., Schifferer, M., Simons, M.
- ID
- ZDB-PUB-230114-6
- Date
- 2023
- Source
- The Journal of cell biology 222(3): (Journal)
- Registered Authors
- Djannatian, Minou Susan, Schmid, Bettina, Simons, Mikael, van Ham, Tjakko
- Keywords
- none
- MeSH Terms
-
- Animals
- Axons/ultrastructure
- Mice
- Microglia*/ultrastructure
- Microscopy, Electron, Scanning
- Myelin Sheath*/ultrastructure
- Oligodendroglia/ultrastructure
- Optic Nerve*/ultrastructure
- Phagocytosis
- Time-Lapse Imaging
- Zebrafish*
- PubMed
- 36637807 Full text @ J. Cell Biol.
Citation
Djannatian, M., Radha, S., Weikert, U., Safaiyan, S., Wrede, C., Deichsel, C., Kislinger, G., Rhomberg, A., Ruhwedel, T., Campbell, D.S., van Ham, T., Schmid, B., Hegermann, J., Möbius, W., Schifferer, M., Simons, M. (2023) Myelination generates aberrant ultrastructure that is resolved by microglia. The Journal of cell biology. 222(3):.
Abstract
To enable rapid propagation of action potentials, axons are ensheathed by myelin, a multilayered insulating membrane formed by oligodendrocytes. Most of the myelin is generated early in development, resulting in the generation of long-lasting stable membrane structures. Here, we explored structural and dynamic changes in central nervous system myelin during development. To achieve this, we performed an ultrastructural analysis of mouse optic nerves by serial block face scanning electron microscopy (SBF-SEM) and confocal time-lapse imaging in the zebrafish spinal cord. We found that myelin undergoes extensive ultrastructural changes during early postnatal development. Myelin degeneration profiles were engulfed and phagocytosed by microglia using exposed phosphatidylserine as one "eat me" signal. In contrast, retractions of entire myelin sheaths occurred independently of microglia and involved uptake of myelin by the oligodendrocyte itself. Our findings show that the generation of myelin early in development is an inaccurate process associated with aberrant ultrastructural features that require substantial refinement.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping