PUBLICATION

A cerebellar-prepontine circuit for tonic immobility triggered by an inescapable threat

Authors
Bhandiwad, A.A., Chu, N.C., Semenova, S.A., Holmes, G.A., Burgess, H.A.
ID
ZDB-PUB-220930-1
Date
2022
Source
Science advances   8: eabo0549 (Journal)
Registered Authors
Burgess, Harold
Keywords
none
MeSH Terms
none
PubMed
36170356 Full text @ Sci Adv
Abstract
Sudden changes in the environment are frequently perceived as threats and provoke defensive behavioral states. One such state is tonic immobility, a conserved defensive strategy characterized by powerful suppression of movement and motor reflexes. Tonic immobility has been associated with multiple brainstem regions, but the underlying circuit is unknown. Here, we demonstrate that a strong vibratory stimulus evokes tonic immobility in larval zebrafish defined by suppressed locomotion and sensorimotor responses. Using a circuit-breaking screen and targeted neuron ablations, we show that cerebellar granule cells and a cluster of glutamatergic ventral prepontine neurons (vPPNs) that express key stress-associated neuropeptides are critical components of the circuit that suppresses movement. The complete sensorimotor circuit transmits information from sensory ganglia through the cerebellum to vPPNs to regulate reticulospinal premotor neurons. These results show that cerebellar regulation of a neuropeptide-rich prepontine structure governs a conserved and ancestral defensive behavior that is triggered by an inescapable threat.
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m1230TgTransgenic Insertion
    nns14TgTransgenic Insertion
      nns26TgTransgenic Insertion
        nub6TgTransgenic Insertion
          s1999tTgTransgenic Insertion
            vo4TgTransgenic Insertion
              y256EtTransgenic Insertion
              y260TgTransgenic Insertion
                y266TgTransgenic Insertion
                  y268TgTransgenic Insertion
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                    Engineered Foreign Genes
                    Marker Marker Type Name
                    CeruleanEFGCerulean
                    CreEFGCre
                    DsRedEFGDsRed
                    DsRed2EFGDsRed2
                    EGFPEFGEGFP
                    GAL4FFEFGGAL4FF
                    GCaMPEFGGCaMP
                    GFPEFGGFP
                    KaedeEFGKaede
                    NTREFGNTR
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                    Citation
                    Bhandiwad, A.A., Chu, N.C., Semenova, S.A., Holmes, G.A., Burgess, H.A. (2022) A cerebellar-prepontine circuit for tonic immobility triggered by an inescapable threat. Science advances. 8:eabo0549.