PUBLICATION

Embryonic Hyperglycemia Delays the Development of Retinal Synapses in a Zebrafish Model

Authors
Shrestha, A.P., Saravanakumar, A., Konadu, B., Madireddy, S., Gibert, Y., Vaithianathan, T.
ID
ZDB-PUB-220911-8
Date
2022
Source
International Journal of Molecular Sciences   23(17): (Journal)
Registered Authors
Gibert, Yann
Keywords
hyperglycemia, retinal development, ribbon synapse, ribeye protein, synaptic proteins, zebrafish (Danio rerio) model
MeSH Terms
  • Animals
  • Hyperglycemia*/metabolism
  • Retina/metabolism
  • Synapses/metabolism
  • Zebrafish*/metabolism
  • Zebrafish Proteins/metabolism
PubMed
36077087 Full text @ Int. J. Mol. Sci.
Abstract
Embryonic hyperglycemia negatively impacts retinal development, leading to abnormal visual behavior, altered timing of retinal progenitor differentiation, decreased numbers of retinal ganglion cells and Müller glia, and vascular leakage. Because synaptic disorganization is a prominent feature of many neurological diseases, the goal of the current work was to study the potential impact of hyperglycemia on retinal ribbon synapses during embryonic development. Our approach utilized reverse transcription quantitative PCR (RT-qPCR) and immunofluorescence labeling to compare the transcription of synaptic proteins and their localization in hyperglycemic zebrafish embryos, respectively. Our data revealed that the maturity of synaptic ribbons was compromised in hyperglycemic zebrafish larvae, where altered ribeye expression coincided with the delay in establishing retinal ribbon synapses and an increase in the immature synaptic ribbons. Our results suggested that embryonic hyperglycemia disrupts retinal synapses by altering the development of the synaptic ribbon, which can lead to visual defects. Future studies using zebrafish models of hyperglycemia will allow us to study the underlying mechanisms of retinal synapse development.
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Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
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Errata and Notes