PUBLICATION
Methylome data derived from maternal-zygotic DNA methyltransferase 3aa-/- zebrafish
- Authors
- Shirai, M., Takayama, K., Takahashi, H., Hirose, Y., Fujii, M., Awazu, A., Shimoda, N., Kikuchi, Y.
- ID
- ZDB-PUB-220831-9
- Date
- 2022
- Source
- Data in brief 44: 108514 (Journal)
- Registered Authors
- Kikuchi, Yutaka, Shimoda, Nobuyoshi
- Keywords
- DNA methylation, Dnmt3aa, Whole genome bisulfate sequencing, Zebrafish
- Datasets
- GEO:GSE178691, GEO:GSE178690, GEO:GSE178688
- MeSH Terms
- none
- PubMed
- 36034642 Full text @ Data Brief
Citation
Shirai, M., Takayama, K., Takahashi, H., Hirose, Y., Fujii, M., Awazu, A., Shimoda, N., Kikuchi, Y. (2022) Methylome data derived from maternal-zygotic DNA methyltransferase 3aa-/- zebrafish. Data in brief. 44:108514.
Abstract
Genomic DNA methylation is an epigenetic marker mediated by DNA methyltransferases (Dnmts); in vertebrates, it comprises of a maintenance DNA methyltransferase, Dnmt1, and two de novo DNA methyltransferases (Dnmt3a and Dnmt3b). In zebrafish, there are two homologs of the mammalian Dnmt3a: Dnmt3aa and Dnmt3ab. A knockout (KO) mutant of zebrafish dnmt3aa was generated using the CRISPR/Cas9 genome-editing system as a new model for DNA methylation research. Since zebrafish dnmt3aa KO mutants were viable and fertile, a maternal-zygotic dnmt3aa deficient mutant (MZdnmt3aa) was generated. We performed whole-genome bisulfite sequencing (WGBS) to reveal the DNA methylation profile using this mutant and identified genomic regions with altered CpG methylation as differentially methylated regions (DMRs) in this mutant compared to those in the wild-type fish. We provided novel raw and processed datasets using the MZdnmt3aa KO mutant, and the raw data of WGBS are available through the Gene Expression Omnibus (GEO), accession number GSE178690.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping