Sema4C Is Required for Vascular and Primary Motor Neuronal Patterning in Zebrafish
- Sheng, J., Jiang, B., Shi, R., Shi, L., Liu, D.
- Cells 11(16): (Journal)
- Registered Authors
- Liu, Dong
- endothelial cells, guidance cues, motor neuron, pathfinding, zebrafish
- MeSH Terms
- Endothelial Cells/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- 36010604 Full text @ Cells
Sheng, J., Jiang, B., Shi, R., Shi, L., Liu, D. (2022) Sema4C Is Required for Vascular and Primary Motor Neuronal Patterning in Zebrafish. Cells. 11(16).
Endothelial cells (ECs) and neurons share a number of common signaling pathways and molecular mediators to orchestrate directional migration and guide the pattern of the vascular network and nervous system. So far, research concerning the functional coupling between vascular and neuronal pathfinding remains insufficient. Semaphorin4C (sema4C), a member of class 4 semaphorins, is initially described in the nervous system, whose role has been demonstrated in diverse biological developments. The present study focused on the role of sema4C in the vascular and neural development process in zebrafish embryos. It confirmed that sema4C is expressed in both the nervous system and intersegmental vessels (ISVs) in zebrafish embryos by diverse expression analysis. It also showed that the knockdown of sema4C caused a serious pathfinding anomaly both in the ISVs and primary motor neurons (PMNs) of zebrafish embryos. In addition, overexpressing exogenous sema4C mRNA in sema4C morphants remarkably neutralized the defective pattern of the vascular and neural system. Collectively, this report suggests that sema4C acts as a dual guiding factor regulating vascular and neuronal development. These findings elucidate a new molecular mechanism underlying blood vessel and nerve development and might serve as groundwork for future research on functional coupling between both systems.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes