PUBLICATION
Investigation Driven by Network Pharmacology on Potential Components and Mechanism of DGS, a Natural Vasoprotective Combination, for the Phytotherapy of Coronary Artery Disease
- Authors
- Zhang, Y.G., Liu, X.X., Zong, J.C., Zhang, Y.T., Dong, R., Wang, N., Ma, Z.H., Li, L., Wang, S.L., Mu, Y.L., Wang, S.S., Liu, Z.M., Han, L.W.
- ID
- ZDB-PUB-220710-12
- Date
- 2022
- Source
- Molecules 27(13): (Journal)
- Registered Authors
- Keywords
- angiogenesis, coronary artery disease, dynamics molecular docking, network pharmacology, phytotherapy, zebrafish
- MeSH Terms
-
- Animals
- Coronary Artery Disease*/drug therapy
- Drugs, Chinese Herbal*
- Molecular Docking Simulation
- Network Pharmacology
- Phosphatidylinositol 3-Kinases/metabolism
- Phytotherapy
- Proto-Oncogene Proteins c-akt/metabolism
- Zebrafish/metabolism
- PubMed
- 35807320 Full text @ Molecules
Citation
Zhang, Y.G., Liu, X.X., Zong, J.C., Zhang, Y.T., Dong, R., Wang, N., Ma, Z.H., Li, L., Wang, S.L., Mu, Y.L., Wang, S.S., Liu, Z.M., Han, L.W. (2022) Investigation Driven by Network Pharmacology on Potential Components and Mechanism of DGS, a Natural Vasoprotective Combination, for the Phytotherapy of Coronary Artery Disease. Molecules. 27(13).
Abstract
Phytotherapy offers obvious advantages in the intervention of Coronary Artery Disease (CAD), but it is difficult to clarify the working mechanisms of the medicinal materials it uses. DGS is a natural vasoprotective combination that was screened out in our previous research, yet its potential components and mechanisms are unknown. Therefore, in this study, HPLC-MS and network pharmacology were employed to identify the active components and key signaling pathways of DGS. Transgenic zebrafish and HUVECs cell assays were used to evaluate the effectiveness of DGS. A total of 37 potentially active compounds were identified that interacted with 112 potential targets of CAD. Furthermore, PI3K-Akt, MAPK, relaxin, VEGF, and other signal pathways were determined to be the most promising DGS-mediated pathways. NO kit, ELISA, and Western blot results showed that DGS significantly promoted NO and VEGFA secretion via the upregulation of VEGFR2 expression and the phosphorylation of Akt, Erk1/2, and eNOS to cause angiogenesis and vasodilation. The result of dynamics molecular docking indicated that Salvianolic acid C may be a key active component of DGS in the treatment of CAD. In conclusion, this study has shed light on the network molecular mechanism of DGS for the intervention of CAD using a network pharmacology-driven strategy for the first time to aid in the intervention of CAD.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping