PUBLICATION

Optic nerve injury-induced regeneration in the adult zebrafish is accompanied by spatiotemporal changes in mitochondrial dynamics

Authors
Beckers, A., Masin, L., Van Dyck, A., Bergmans, S., Vanhunsel, S., Zhang, A., Verreet, T., Poulain, F.E., Farrow, K., Moons, L.
ID
ZDB-PUB-220709-4
Date
2023
Source
Neural regeneration research   18: 219-225 (Journal)
Registered Authors
Poulain, Fabienne
Keywords
axonal regeneration, central nervous system, dendrite remodeling, energy metabolism, fission, mitochondria, mitochondrial trafficking, optic nerve crush, retina, zebrafish
MeSH Terms
none
PubMed
35799546 Full text @ Neural Regen Res
Abstract
Axonal regeneration in the central nervous system is an energy-intensive process. In contrast to mammals, adult zebrafish can functionally recover from neuronal injury. This raises the question of how zebrafish can cope with this high energy demand. We previously showed that in adult zebrafish, subjected to an optic nerve crush, an antagonistic axon-dendrite interplay exists wherein the retraction of retinal ganglion cell dendrites is a prerequisite for effective axonal repair. We postulate a 'dendrites for regeneration' paradigm that might be linked to intraneuronal mitochondrial reshuffling, as ganglion cells likely have insufficient resources to maintain dendrites and restore axons simultaneously. Here, we characterized both mitochondrial distribution and mitochondrial dynamics within the different ganglion cell compartments (dendrites, somas, and axons) during the regenerative process. Optic nerve crush resulted in a reduction of mitochondria in the dendrites during dendritic retraction, whereafter enlarged mitochondria appeared in the optic nerve/tract during axonal regrowth. Upon dendritic regrowth in the retina, mitochondrial density inside the retinal dendrites returned to baseline levels. Moreover, a transient increase in mitochondrial fission and biogenesis was observed in retinal ganglion cell somas after optic nerve damage. Taken together, these findings suggest that during optic nerve injury-induced regeneration, mitochondria shift from the dendrites to the axons and back again and that temporary changes in mitochondrial dynamics support axonal and dendritic regrowth after optic nerve crush.
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Allele Construct Type Affected Genomic Region
usc4TgTransgenic Insertion
    usc5TgTransgenic Insertion
      usc6TgTransgenic Insertion
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        Name Type Antigen Genes Isotypes Host Organism
        Ab1-chatpolyclonalGoat
        Ab2-dnm1lmonoclonal
          		IgGRabbit
          Ab3-ppargc1amonoclonal
            		IgG2aMouse
            Ab5-pink1polyclonal
              		Rabbit
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              Marker Marker Type Name
              EGFPEFGEGFP
              TagBFPEFGTagBFP
              TagRFPEFGTagRFP
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              Citation
              Beckers, A., Masin, L., Van Dyck, A., Bergmans, S., Vanhunsel, S., Zhang, A., Verreet, T., Poulain, F.E., Farrow, K., Moons, L. (2023) Optic nerve injury-induced regeneration in the adult zebrafish is accompanied by spatiotemporal changes in mitochondrial dynamics. Neural regeneration research. 18:219-225.