PUBLICATION
Midnight/midday-synchronized expression of cryptochrome genes in the eyes of three teleost species, zebrafish, goldfish, and medaka
- Authors
- Nakagawa, M., Okano, K., Saratani, Y., Shoji, Y., Okano, T.
- ID
- ZDB-PUB-220609-2
- Date
- 2022
- Source
- Zoological letters 8: 8 (Journal)
- Registered Authors
- Keywords
- Circadian clock, Cryptochrome, Eye, Goldfish, Medaka, Photoperiodicity, Quantitative RT-PCR, Sun compass, Zebrafish
- MeSH Terms
- none
- PubMed
- 35672786 Full text @ Zoological Lett
Citation
Nakagawa, M., Okano, K., Saratani, Y., Shoji, Y., Okano, T. (2022) Midnight/midday-synchronized expression of cryptochrome genes in the eyes of three teleost species, zebrafish, goldfish, and medaka. Zoological letters. 8:8.
Abstract
Photoperiodic responses are observed in many organisms living in the temperate zones. The circadian clock is involved in photoperiodic time measurement; however, the underlying molecular mechanism for detection of the day length remains unknown. We previously compared the expression profiles of the Cryptochrome(Cry) genes in the zebrafish eye and reported that Cry1ab has a double peak with variable expression duration depending on the photoperiod. In this study, to understand commonalities and differences in the photoperiodic responses of ocular Cry genes, we identified Cryptochrome genes in two other teleost species, goldfish and medaka, living in temperate zones, and measured ocular Cry mRNA levels in all of the three species, under different photoperiods (long-day [14 h light: 10 h dark] and short-day [10 h light: 14 h dark] and in constant darkness. Cry1ab mRNA levels did not show dual peaks in goldfish or medaka under the examined conditions; however, the mRNA expression profiles of many Crys were altered in all three species, depending on the day length and light condition. Based on their expression profiles, Cry mRNA peaks were classified into three groups that better synchronize to sunrise (light-on), midnight/midday (middle points of the dark/light periods), or sunset (light-off). These results suggest the presence of multiple oscillators that oscillate independently or a complex oscillator in which Cry expression cycles change in a photoperiod-dependent manner in the eye.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping