PUBLICATION
Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium
- Authors
- Mytlis, A., Kumar, V., Qiu, T., Deis, R., Hart, N., Levy, K., Masek, M., Shawahny, A., Ahmad, A., Eitan, H., Nather, F., Adar-Levor, S., Birnbaum, R.Y., Elia, N., Bachmann-Gagescu, R., Roy, S., Elkouby, Y.M.
- ID
- ZDB-PUB-220514-11
- Date
- 2022
- Source
- Science (New York, N.Y.) 376(6599): eabh3104 (Journal)
- Registered Authors
- Bachmann-Gagescu, Ruxandra, Birnbaum, Ramon, Elkouby, Yaniv M., Masek, Markus, Roy, Sudipto
- Keywords
- none
- MeSH Terms
-
- Animals
- Centromere/genetics
- Centromere/physiology
- Chromosome Pairing*/genetics
- Chromosome Pairing*/physiology
- Cilia*/physiology
- Female
- Fertility/physiology
- Mice
- Morphogenesis
- Oocytes*/growth & development
- Oogenesis*/genetics
- Oogenesis*/physiology
- Ovary*/growth & development
- Telomere/genetics
- Telomere/physiology
- Zebrafish/genetics
- Zebrafish/physiology
- PubMed
- 35549308 Full text @ Science
Citation
Mytlis, A., Kumar, V., Qiu, T., Deis, R., Hart, N., Levy, K., Masek, M., Shawahny, A., Ahmad, A., Eitan, H., Nather, F., Adar-Levor, S., Birnbaum, R.Y., Elia, N., Bachmann-Gagescu, R., Roy, S., Elkouby, Y.M. (2022) Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium. Science (New York, N.Y.). 376(6599):eabh3104.
Abstract
A hallmark of meiosis is chromosomal pairing, which requires telomere tethering and rotation on the nuclear envelope via microtubules, driving chromosome homology searches. Telomere pulling toward the centrosome forms the "zygotene chromosomal bouquet". Here, we identified the "zygotene cilium" in oocytes. This cilium provides a cable system for the bouquet machinery, extending throughout the germline cyst. Using zebrafish mutants and live manipulations, we demonstrate that the cilium anchors the centrosome to counterbalance telomere pulling. The cilium is essential for bouquet and synaptonemal complex formation, oogenesis, ovarian development, and fertility. Thus, a cilium represents a conserved player in zebrafish and mouse meiosis, which sheds light on reproductive aspects in ciliopathies, and suggests that cilia can control chromosomal dynamics.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping