PUBLICATION
The distal C terminus of the dihydropyridine receptor β1a subunit is essential for tetrad formation in skeletal muscle
- Authors
- Dayal, A., Perni, S., Franzini-Armstrong, C., Beam, K.G., Grabner, M.
- ID
- ZDB-PUB-220506-9
- Date
- 2022
- Source
- Proceedings of the National Academy of Sciences of the United States of America 119: e2201136119 (Journal)
- Registered Authors
- Dayal, Anamika, Grabner, Manfred
- Keywords
- excitation–contraction coupling, skeletal muscle, tetrad formation, voltage-gated Ca2+ channel, β subunit
- MeSH Terms
-
- Adaptor Proteins, Signal Transducing
- Animals
- Calcium/metabolism
- Calcium Channels, L-Type*/metabolism
- Excitation Contraction Coupling
- Mice
- Muscle Fibers, Skeletal*/metabolism
- Muscle, Skeletal/metabolism
- Ryanodine Receptor Calcium Release Channel*/metabolism
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 35507876 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Dayal, A., Perni, S., Franzini-Armstrong, C., Beam, K.G., Grabner, M. (2022) The distal C terminus of the dihydropyridine receptor β1a subunit is essential for tetrad formation in skeletal muscle. Proceedings of the National Academy of Sciences of the United States of America. 119:e2201136119.
Abstract
SignificanceVertebrate skeletal muscle excitation-contraction coupling (ECC) is based on Ca2+-influx-independent interchannel cross-talk between DHPR and RyR1. The skeletal muscle DHPR complex consists of the main, voltage-sensing, and pore-forming α1S subunit, the auxiliary β1a, α2δ-1, γ1 subunits, and Stac3. The DHPRβ1a subunit plays an essential role in full triad targeting of DHPRα1S, voltage sensing, and tetrad formation (grouping of four DHPRs)-the three prerequisites for skeletal muscle ECC. Hence, a lack of DHPRβ1a results in a lethal phenotype in both β1-null mice and zebrafish. Here, we identified the nonconserved, distal C terminus of DHPRβ1a as playing a pivotal role in the formation of DHPR tetrads, and thus allosteric DHPR-RyR1 coupling, essential for proper skeletal muscle ECC.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping