PUBLICATION
Slc38a9 Deficiency Induces Apoptosis and Metabolic Dysregulation and Leads to Premature Death in Zebrafish
- Authors
- Wu, X., Chen, J., Liu, C., Wang, X., Zhou, H., Mai, K., He, G.
- ID
- ZDB-PUB-220424-16
- Date
- 2022
- Source
- International Journal of Molecular Sciences 23(8): (Journal)
- Registered Authors
- Liu, Chengdong
- Keywords
- SLC38A9, amino acid homeostasis, apoptosis, glycolysis, hypoxia
- MeSH Terms
-
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Mortality, Premature*
- Amino Acid Transport Systems/metabolism
- Animals
- Apoptosis/genetics
- Amino Acids/metabolism
- PubMed
- 35457018 Full text @ Int. J. Mol. Sci.
Citation
Wu, X., Chen, J., Liu, C., Wang, X., Zhou, H., Mai, K., He, G. (2022) Slc38a9 Deficiency Induces Apoptosis and Metabolic Dysregulation and Leads to Premature Death in Zebrafish. International Journal of Molecular Sciences. 23(8):.
Abstract
Eukaryotic cells control nutritional homeostasis and determine cell metabolic fate through a series of nutrient transporters and metabolic regulation pathways. Lysosomal localized amino acid transporter member 9 of the solute carrier family 38 (SLC38A9) regulates essential amino acids' efflux from lysosomes in an arginine-regulated fashion. To better understand the physiological role of SLC38A9, we first described the spatiotemporal expression pattern of the slc38a9 gene in zebrafish. A quarter of slc38a9-/- mutant embryos developed pericardial edema and died prematurely, while the remaining mutants were viable and grew normally. By profiling the transcriptome of the abnormally developed embryos using RNA-seq, we identified increased apoptosis, dysregulated amino acid metabolism, and glycolysis/gluconeogenesis disorders that occurred in slc38a9-/- mutant fish. slc38a9 deficiency increased whole-body free amino acid and lactate levels but reduced glucose and pyruvate levels. The change of glycolysis-related metabolites in viable slc38a9-/- mutant fish was ameliorated. Moreover, loss of slc38a9 resulted in a significant reduction in hypoxia-inducible gene expression and hypoxia-inducible factor 1-alpha (Hif1α) protein levels. These results improved our understanding of the physiological functions of SLC38A9 and revealed its indispensable role in embryonic development, metabolic regulation, and stress adaption.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping