PUBLICATION

Colorimetric and fluorescent TRAP assays for visualising and quantifying fish osteoclast activity

Authors
Ethiraj, L.P., Fong, E.L.S., Liu, R., Chan, M., Winkler, C., Carney, T.J.
ID
ZDB-PUB-220326-7
Date
2022
Source
European journal of histochemistry : EJH   66(2): (Journal)
Registered Authors
Carney, Tom, Winkler, Christoph
Keywords
none
MeSH Terms
  • Acid Phosphatase*/analysis
  • Animals
  • Colorimetry
  • Isoenzymes
  • Osteoclasts*/chemistry
  • Tartrate-Resistant Acid Phosphatase/analysis
  • Zebrafish
PubMed
35330553 Full text @ Eur. J. Histochem.
Abstract
Histochemical detection of tartrate-resistant acid phosphatase (TRAP) activity is a fundamental technique for visualizing osteoclastic bone resorption and assessing osteoclast activity status in tissues. This approach has mostly employed colorimetric detection, which has limited quantification of activity in situ and co-labelling with other skeletal markers. Here we report simple colorimetric and fluorescent TRAP assays in zebrafish and medaka, two important model organisms for investigating the pathogenesis of bone disorders. We show fluorescent TRAP staining, utilising the ELF97 substrate, is a rapid, robust and stable system to visualise and quantify osteoclast activity in zebrafish, and is compatible with other fluorescence stains, transgenic lines and antibody approaches. Using this approach, we show that TRAP activity is predominantly found around the base of the zebrafish pharyngeal teeth, where osteoclast activity state appears to be heterogeneous.
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Human Disease / Model
Sequence Targeting Reagents
Fish
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