PUBLICATION
Spironolactone affects cardiovascular and craniofacial development in zebrafish embryos (Danio rerio)
- Authors
- Petty, H.J., Barrett, J.E., Kosmowski, E.G., Amos, D.S., Ryan, S.M., Jones, L.D., Lassiter, C.S.
- ID
- ZDB-PUB-220316-5
- Date
- 2022
- Source
- Environmental Toxicology and Pharmacology 92: 103848 (Journal)
- Registered Authors
- Lassiter, Christopher S.
- Keywords
- cardiovascular, cartilage, craniofacial, spironolactone, zebrafish
- MeSH Terms
-
- Animals
- Dihydrotachysterol
- Diuretics
- Embryonic Development
- Humans
- Spironolactone*/pharmacology
- Zebrafish*
- PubMed
- 35288337 Full text @ Environ. Toxicol. Pharmacol.
Citation
Petty, H.J., Barrett, J.E., Kosmowski, E.G., Amos, D.S., Ryan, S.M., Jones, L.D., Lassiter, C.S. (2022) Spironolactone affects cardiovascular and craniofacial development in zebrafish embryos (Danio rerio). Environmental Toxicology and Pharmacology. 92:103848.
Abstract
Spironolactone, a potassium-sparing diuretic and aldosterone antagonist, is a mineralocorticoid hormone commonly prescribed to patients suffering from heart failure, hirsutism, dermatological afflictions, and hypertension. Interestingly, relatively little work has been done on the development of vertebrate embryos after exposure to this compound. Here, we treat zebrafish embryos with spironolactone at 10-6M, 10-7M, or 10-8M, and observe them after three to seven days of exposure. While no effect was observed in mortality, we did detect differences in cardiovascular development at 3 dpf and craniofacial development at 5 dpf. At 10-6M, smaller atria, ventricles, and blood vessels were observed. The highest concentrations also caused a longer ceratohyal/Meckel's distance, longer palatoquadrate, and smaller angles between the palatoquadrate and both the ceratohyal and Meckel's. Further research of spironolactone's effects on embryonic development could lead to a better understanding of the compound resulting in improved public and environmental health.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping