PUBLICATION
Contingent stimulus delivery assay for zebrafish reveals a role for CCSER1 in alcohol preference
- Authors
- Nathan, F.M., Kibat, C., Goel, T., Stewart, J., Claridge-Chang, A., Mathuru, A.S.
- ID
- ZDB-PUB-220303-17
- Date
- 2022
- Source
- Addiction biology 27: e13126 (Journal)
- Registered Authors
- Mathuru, Ajay
- Keywords
- addiction, alcohol, alcohol use disorder, hormesis, self-administration, zebrafish
- MeSH Terms
-
- Alcoholism*/genetics
- Animals
- Behavior, Addictive*
- Ethanol/pharmacology
- Humans
- Self Administration
- Zebrafish
- PubMed
- 35229935 Full text @ Addict Biol
Citation
Nathan, F.M., Kibat, C., Goel, T., Stewart, J., Claridge-Chang, A., Mathuru, A.S. (2022) Contingent stimulus delivery assay for zebrafish reveals a role for CCSER1 in alcohol preference. Addiction biology. 27:e13126.
Abstract
Alcohol use disorders are complex, multifactorial phenomena with a large footprint within the global burden of diseases. Here, we report the development of an accessible, two-choice self-administration zebrafish assay (SAZA) to study the neurobiology of addiction. Using this assay, we first demonstrated that, although zebrafish avoid higher concentrations of alcohol, they are attracted to low concentrations. Pre-exposure to alcohol did not change this relative preference, but acute exposure to an alcohol deterrent approved for human use decreased alcohol self-administration. A pigment mutant used in whole-brain imaging studies displayed a similar relative alcohol preference profile; however, mutants in CCSER1, a gene associated with alcohol dependence in human genetic studies, showed a reversal in relative preference. The presence of a biphasic response (hormesis) in zebrafish validated a key aspect of vertebrate responses to alcohol. SAZA adds a new dimension for discovering novel alcohol deterrents and studying the neurogenetics of addiction using the zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping