PUBLICATION
Bifidobacterium lactis BL-99 modulates intestinal inflammation and functions in zebrafish models
- Authors
- Chen, M., Liu, C., Dai, M., Wang, Q., Li, C., Hung, W.
- ID
- ZDB-PUB-220217-5
- Date
- 2022
- Source
- PLoS One 17: e0262942 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Alum Compounds/toxicity
- Animals
- Bifidobacterium/physiology*
- Constipation/chemically induced
- Constipation/pathology
- Constipation/therapy
- Discriminant Analysis
- Disease Models, Animal
- Down-Regulation/drug effects
- Gastrointestinal Motility/drug effects
- Glucose/pharmacology
- Inflammation/chemically induced
- Inflammation/pathology
- Inflammation/therapy*
- Interleukin-10/genetics
- Interleukin-10/metabolism
- Interleukin-1beta/genetics
- Interleukin-1beta/metabolism
- Intestines/metabolism*
- Intestines/microbiology
- Intestines/pathology
- Larva/drug effects
- Larva/metabolism
- Probiotics/pharmacology
- Probiotics/therapeutic use*
- Up-Regulation/drug effects
- Zebrafish/growth & development
- PubMed
- 35171916 Full text @ PLoS One
Citation
Chen, M., Liu, C., Dai, M., Wang, Q., Li, C., Hung, W. (2022) Bifidobacterium lactis BL-99 modulates intestinal inflammation and functions in zebrafish models. PLoS One. 17:e0262942.
Abstract
This study was designed to explore the therapeutics and the mechanisms of a patented and marked gastric acid and intestine juice-resistant probiotics Bifidobacterium lactis BL-99 (B. lactis BL-99) on the intestinal inflammation and functions in the zebrafish models. After feeding for 6 hours, B. lactis BL-99 was fully retained in the larval zebrafish intestinal tract and stayed for over 24 hours. B. lactis BL-99 promoted the intestinal motility and effectively alleviated aluminum sulfate-induced larval zebrafish constipation (p < 0.01). Irregular high glucose diet induced adult zebrafish intestinal functional and metabolic disorders. After fed with B. lactis BL-99, IL-1β gene expression was significantly down-regulated, and IL-10 and IL-12 gene levels were markedly up-regulated in this model (p < 0.05). The intestinal lipase activity was elevated in the adult zebrafish intestinal functional disorder model after B. lactis BL-99 treatment (p < 0.05), but tryptase content had no statistical changes (p > 0.05). B. lactis BL-99 improved the histopathology of the adult zebrafish intestinal inflammation, increased the goblet cell numbers, and up-and-down metabolites were markedly recovered after treatment of B. lactis BL-99 (p < 0.05). These results suggest that B. lactis BL-99 could relieve intestinal inflammation and promote intestinal functions, at least in part, through modulating intestinal and microbial metabolism to maintain intestinal health.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping