PUBLICATION
Pro-Angiogenic Effects of Essential Oil from Perilla frutescens and Its Main Component (Perillaldehyde) on Zebrafish Embryos and Human Umbilical Vein Endothelial Cells
- Authors
- Zhou, F., Dai, O., Peng, C., Xiong, L., Ao, H., Liu, F., Zhou, Q.M.
- ID
- ZDB-PUB-211221-28
- Date
- 2021
- Source
- Drug design, development and therapy 15: 4985-4999 (Journal)
- Registered Authors
- Keywords
- HUVECs, Perilla frutescens, essential oil, perillaldehyde, pro-angiogenesis, zebrafish
- MeSH Terms
-
- Angiogenesis Inducing Agents/pharmacology*
- Animals
- Embryo, Nonmammalian/drug effects*
- Human Umbilical Vein Endothelial Cells/drug effects*
- Humans
- Monoterpenes/pharmacology*
- Oils, Volatile
- Perilla frutescens*
- Zebrafish
- PubMed
- 34924753 Full text @ Drug Des Devel Ther
Citation
Zhou, F., Dai, O., Peng, C., Xiong, L., Ao, H., Liu, F., Zhou, Q.M. (2021) Pro-Angiogenic Effects of Essential Oil from Perilla frutescens and Its Main Component (Perillaldehyde) on Zebrafish Embryos and Human Umbilical Vein Endothelial Cells. Drug design, development and therapy. 15:4985-4999.
Abstract
Purpose Perilla frutescens (L.) Britt., a traditional edible-medicinal herb in China, has been used to treat cardiovascular and cerebrovascular (cardio-cerebrovascular) diseases for thousands of years. However, knowledge of the mechanisms underlying the effects of essential oil from P. frutescens (EOPF) in the treatment of cardio-cerebrovascular diseases is lacking. The promotion of angiogenesis is beneficial in the treatment of ischemic cardio-cerebrovascular diseases. The current study investigated the pro-angiogenic role of EOPF and its main component perillaldehyde in sunitinib-injured transgenic Tg (flk1:EGFP) zebrafish embryos and human umbilical vein endothelial cells (HUVECs) for the first time.
Materials and methods The pro-angiogenic effects of EOPF and perillaldehyde were observed in vivo using transgenic Tg (flk1:EGFP) zebrafish embryos and in vitro using HUVECs. Cell viability, proliferation, migration, tube formation, and protein levels were detected by MTT, EdU staining, wound healing, transwell chamber, and Western blot assays, respectively.
Results EOPF and perillaldehyde exerted a significant stimulatory effect on the formation of zebrafish intersegmental vessels (ISVs). Moreover, EOPF and perillaldehyde promoted proliferation, migration, and tube formation in sunitinib-treated HUVECs. Additionally, our findings uncovered that the pro-angiogenic effects of EOPF and perillaldehyde were mediated by increases in the expression ratios of p-ERK1/2 to ERK1/2 and Bcl-2 to Bax.
Conclusion The present study is the first report to provide clear evidence that EOPF and perillaldehyde promote angiogenesis by stimulating repair of sunitinib-injured ISVs in zebrafish embryos and promoting proliferation, migration, and tube formation in sunitinib-injured HUVECs. The underlying mechanisms are related to increased p-ERK1/2 to ERK1/2 and Bcl-2 to Bax expression ratios. EOPF and perillaldehyde may be used in the treatment of cardio-cerebrovascular diseases, which is consistent with the traditional application of P. frutescens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping