PUBLICATION
The Anti-Tumoral Potential of Phosphonate Analog of Sulforaphane in Zebrafish Xenograft Model
- Authors
- Rudzinska-Radecka, M., Janczewski, Ł., Gajda, A., Godlewska, M., Chmielewska-Krzesinska, M., Wasowicz, K., Podlasz, P.
- ID
- ZDB-PUB-211129-64
- Date
- 2021
- Source
- Cells 10(11): (Journal)
- Registered Authors
- Podlasz, Piotr, Wasowicz, Krzysztof
- Keywords
- breast cancer, cervical cancer, glioblastoma, isothiocyanates, toxicology, zebrafish, zebrafish xenograft model
- MeSH Terms
-
- Animals
- Antineoplastic Agents/pharmacology*
- Cell Line, Tumor
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Humans
- Isothiocyanates/chemical synthesis
- Isothiocyanates/chemistry
- Isothiocyanates/pharmacology*
- Microwaves
- Organophosphonates/chemical synthesis
- Organophosphonates/chemistry
- Organophosphonates/pharmacology*
- Signal Transduction/drug effects
- Sulfoxides/chemical synthesis
- Sulfoxides/chemistry
- Sulfoxides/pharmacology*
- Xenograft Model Antitumor Assays*
- Zebrafish/embryology
- Zebrafish/physiology*
- PubMed
- 34831440 Full text @ Cells
Citation
Rudzinska-Radecka, M., Janczewski, Ł., Gajda, A., Godlewska, M., Chmielewska-Krzesinska, M., Wasowicz, K., Podlasz, P. (2021) The Anti-Tumoral Potential of Phosphonate Analog of Sulforaphane in Zebrafish Xenograft Model. Cells. 10(11):.
Abstract
Isothiocyanates (ITCs) show strong activity against numerous human tumors. Five structurally diverse ITCs were tested in vivo using the zebrafish embryos 6 and 48 h post-fertilization (hpf). The survival rate, hatching time, and gross morphological changes were assessed 24, 48, and 72 h after treatment with all compounds in various doses (1-10 µM). As a result, we selected a phosphonate analog of sulforaphane (P-ITC; 1-3 µM) as a non-toxic treatment for zebrafish embryos, both 6 and 48 hpf. Furthermore, the in vivo anti-cancerogenic studies with selected 3 µM P-ITC were performed using a set of cell lines derived from the brain (U87), cervical (HeLa), and breast (MDA-MB-231) tumors. For the experiment, cells were labeled using red fluorescence dye Dil (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine, 10 μg/mL) and injected into the hindbrain ventricle, yolk sac region and Cuvier duct of zebrafish embryos. The tumor size measurement after 48 h of treatment demonstrated the significant inhibition of cancer cell growth in all tested cases by P-ITC compared to the non-treated controls. Our studies provided evidence for P-ITC anti-cancerogenic properties with versatile activity against different cancer types. Additionally, P-ITC demonstrated the safety of use in the living organism at various stages of embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping