PUBLICATION
An ATX-LPA6-Gα13-ROCK axis shapes and maintains caudal vein plexus in zebrafish
- Authors
- Okasato, R., Kano, K., Kise, R., Inoue, A., Fukuhara, S., Aoki, J.
- ID
- ZDB-PUB-211111-1
- Date
- 2021
- Source
- iScience 24: 103254 (Journal)
- Registered Authors
- Keywords
- Cell biology, Developmental biology
- MeSH Terms
- none
- PubMed
- 34755093 Full text @ iScience
Citation
Okasato, R., Kano, K., Kise, R., Inoue, A., Fukuhara, S., Aoki, J. (2021) An ATX-LPA6-Gα13-ROCK axis shapes and maintains caudal vein plexus in zebrafish. iScience. 24:103254.
Abstract
Lysophosphatidic acid (LPA) is a potential regulator of vascular formation derived from blood. In this study, we utilized zebrafish as a model organism to monitor the blood vessel formation in detail. Zebrafish mutant of ATX, an LPA-producing enzyme, had a defect in the caudal vein plexus (CVP). Pharmacological inhibition of ATX resulted in a fusion of the delicate vessels in the CVP to form large sac-like vessels. Mutant embryos of LPA6 receptor and downstream Gα13 showed the same phenotype. Administration of OMPT, a stable LPA-analog, induced rapid CVP constriction, which was attenuated significantly in the LPA6 mutant. We also found that blood flow-induced CVP formation was dependent on ATX. The present study demonstrated that the ATX-LPA6 axis acts cooperatively with blood flow and contributes to the formation and maintenance of the CVP by generating contractive force in endothelial cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping