PUBLICATION

Nxhl Controls Angiogenesis by Targeting VE-PTP Through Interaction With Nucleolin

Authors
Luo, H., Zhang, Y., Deng, Y., Li, L., Sheng, Z., Yu, Y., Lin, Y., Chen, X., Feng, P.
ID
ZDB-PUB-211105-3
Date
2021
Source
Frontiers in cell and developmental biology   9: 728821 (Journal)
Registered Authors
Keywords
VE-PTP, angiogenesis, nucleolin, nxhl, vascular
MeSH Terms
none
PubMed
34733844 Full text @ Front Cell Dev Biol
Abstract
Precise regulation of angiogenesis is required for organ development, wound repair, and tumor progression. Here, we identified a novel gene, nxhl (New XingHuo light), that is conserved in vertebrates and that plays a crucial role in vascular integrity and angiogenesis. Bioinformatic analysis uncovered its essential roles in development based on co-expression with several key developmental genes. Knockdown of nxhl in zebrafish causes global and pericardial edema, loss of blood circulation, and vascular defects characterized by both reduced vascularization in intersegmental vessels and decreased sprouting in the caudal vein plexus. The nxhl gene also affects human endothelial cell behavior in vitro. We found that nxhl functions in part by targeting VE-PTP through interaction with NCL (nucleolin). Loss of ptprb (a VE-PTP ortholo) in zebrafish resulted in defects similar to nxhl knockdown. Moreover, nxhl deficiency attenuates tumor invasion and proteins (including VE-PTP and NCL) associated with angiogenesis and EMT. These findings illustrate that nxhl can regulate angiogenesis via a novel nxhl-NCL-VE-PTP axis, providing a new therapeutic target for modulating vascular formation and function, especially for cancer treatment.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
y1TgTransgenic Insertion
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    ptprbMO4-ptprbMRPHLNO
    ptprbMO5-ptprbMRPHLNO
    zgc:113227MO1-zgc:113227MRPHLNO
    zgc:113227MO2-zgc:113227MRPHLNO
    1 - 4 of 4
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    Fish
    No data available
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
    1 - 1 of 1
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    Mapping
    No data available
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    Citation
    Luo, H., Zhang, Y., Deng, Y., Li, L., Sheng, Z., Yu, Y., Lin, Y., Chen, X., Feng, P. (2021) Nxhl Controls Angiogenesis by Targeting VE-PTP Through Interaction With Nucleolin. Frontiers in cell and developmental biology. 9:728821.