PUBLICATION

Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish

Authors
Phatak, M., Kulkarni, S., Miles, L.B., Anjum, N., Dworkin, S., Sonawane, M.
ID
ZDB-PUB-210928-19
Date
2021
Source
PLoS Genetics   17: e1009823 (Journal)
Registered Authors
Dworkin, Seb, Miles, Lee, Sonawane, Mahendra
Keywords
none
Datasets
GEO:GSE173649
MeSH Terms
  • Animals
  • Endocytosis*
  • Epidermal Cells/cytology
  • Epidermal Cells/metabolism*
  • Mutation
  • Stress, Physiological*
  • Transcriptome
  • Up-Regulation
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
(all 12)
PubMed
34570762 Full text @ PLoS Genet.
Abstract
Epithelia such as epidermis cover large surfaces and are crucial for survival. Maintenance of tissue homeostasis by balancing cell proliferation, cell size, and cell extrusion ensures epidermal integrity. Although the mechanisms of cell extrusion are better understood, how epithelial cells that round up under developmental or perturbed genetic conditions are reintegrated in the epithelium to maintain homeostasis remains unclear. Here, we performed live imaging in zebrafish embryos to show that epidermal cells that round up due to membrane homeostasis defects in the absence of goosepimples/myosinVb (myoVb) function, are reintegrated into the epithelium. Transcriptome analysis and genetic interaction studies suggest that the transcription factor Grainyhead-like 3 (Grhl3) induces the retention of rounded cells by regulating E-cadherin levels. Moreover, Grhl3 facilitates the survival of MyoVb deficient embryos by regulating cell adhesion, cell retention and epidermal architecture. Our analyses have unraveled a mechanism of retention of rounded cells and its importance in epithelial homeostasis.
Genes / Markers
Marker Marker Type Name
cdh1GENEcadherin 1, type 1, E-cadherin (epithelial)
cldn3dGENEclaudin 3d
cldn7bGENEclaudin 7b
cldnaGENEclaudin a
cldnbGENEclaudin b
cldneGENEclaudin e
dsc2lGENEdesmocollin 2 like
grhl1GENEgrainyhead-like transcription factor 1
grhl2aGENEgrainyhead-like transcription factor 2a
grhl3GENEgrainyhead-like transcription factor 3
1 - 10 of 16
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Figures
Figure Gallery (7 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
t30335
    Unknown
    t30505
      Unknown
      t30834
        Point Mutation
        t30835
          Point Mutation
          tt221
            Point Mutation
            zf106TgTransgenic Insertion
              zf3707
                Small Deletion
                1 - 7 of 7
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Target Reagent Reagent Type
                grhl3CRISPR2-grhl3CRISPR
                grhl3MO1-grhl3MRPHLNO
                grhl3MO2-grhl3MRPHLNO
                grhl3MO3-grhl3MRPHLNO
                grhl3MO4-grhl3MRPHLNO
                myo5bMO1-myo5bMRPHLNO
                1 - 6 of 6
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                Fish
                Antibodies
                Orthology
                Engineered Foreign Genes
                Marker Marker Type Name
                EGFPEFGEGFP
                1 - 1 of 1
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                Mapping