PUBLICATION
Co-Treatment of Copper Oxide Nanoparticle and Carbofuran Enhances Cardiotoxicity in Zebrafish Embryos
- Authors
- Saputra, F., Uapipatanakul, B., Lee, J.S., Hung, S.M., Huang, J.C., Pang, Y.C., Muñoz, J.E.R., Macabeo, A.P.G., Chen, K.H., Hsiao, C.D.
- ID
- ZDB-PUB-210808-6
- Date
- 2021
- Source
- International Journal of Molecular Sciences 22(15): (Journal)
- Registered Authors
- Keywords
- CuO nanoparticle, carbofuran, cardiotoxicity, molecular docking, zebrafish
- MeSH Terms
-
- Acetylcholinesterase/chemistry
- Acetylcholinesterase/metabolism
- Animals
- Binding Sites
- Carbofuran/pharmacology
- Carbofuran/toxicity*
- Cardiotoxicity
- Cholinesterase Inhibitors/toxicity*
- Copper/toxicity*
- Drug Synergism
- Heart/drug effects*
- Metal Nanoparticles/toxicity*
- Pesticides/pharmacology
- Pesticides/toxicity*
- Protein Binding
- Zebrafish
- PubMed
- 34361024 Full text @ Int. J. Mol. Sci.
Citation
Saputra, F., Uapipatanakul, B., Lee, J.S., Hung, S.M., Huang, J.C., Pang, Y.C., Muñoz, J.E.R., Macabeo, A.P.G., Chen, K.H., Hsiao, C.D. (2021) Co-Treatment of Copper Oxide Nanoparticle and Carbofuran Enhances Cardiotoxicity in Zebrafish Embryos. International Journal of Molecular Sciences. 22(15):.
Abstract
The use of chemicals to boost food production increases as human consumption also increases. The insectidal, nematicidal and acaricidal chemical carbofuran (CAF), is among the highly toxic carbamate pesticide used today. Alongside, copper oxide nanoparticles (CuO) are also used as pesticides due to their broad-spectrum antimicrobial activity. The overuse of these pesticides may lead to leaching into the aquatic environments and could potentially cause adverse effects to aquatic animals. The aim of this study is to assess the effects of carbofuran and copper oxide nanoparticles into the cardiovascular system of zebrafish and unveil the mechanism behind them. We found that a combination of copper oxide nanoparticle and carbofuran increases cardiac edema in zebrafish larvae and disturbs cardiac rhythm of zebrafish. Furthermore, molecular docking data show that carbofuran inhibits acetylcholinesterase (AChE) activity in silico, thus leading to impair cardiac rhythms. Overall, our data suggest that copper oxide nanoparticle and carbofuran combinations work synergistically to enhance toxicity on the cardiovascular performance of zebrafish larvae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping