PUBLICATION
Characterization and bioactivities of phospholipids from squid viscera and gonads using ultra-performance liquid chromatography-Q-exactive orbitrap/mass spectrometry-based lipidomics and zebrafish models
- Authors
- Li, P., Zhang, M., Xie, D., Zhang, X., Zhang, S., Gao, F., Wang, Y., Hsiao, C.D., Li, X., Liu, K.
- ID
- ZDB-PUB-210715-3
- Date
- 2021
- Source
- Food & function 12(17): 7986-7996 (Journal)
- Registered Authors
- Hsiao, Chung-Der
- Keywords
- none
- MeSH Terms
-
- Mass Spectrometry
- Gonads/chemistry
- Humans
- Arrhythmias, Cardiac/drug therapy
- Decapodiformes/chemistry*
- Thrombosis/drug therapy
- Lipidomics
- Animals
- Chromatography, High Pressure Liquid
- Zebrafish
- Fibrinolytic Agents/administration & dosage
- Fibrinolytic Agents/chemistry
- Viscera/chemistry
- Antioxidants/administration & dosage
- Antioxidants/chemistry
- Anti-Inflammatory Agents/administration & dosage
- Anti-Inflammatory Agents/chemistry
- Phospholipids/administration & dosage
- Phospholipids/chemistry*
- Cardiovascular Agents/administration & dosage
- Cardiovascular Agents/chemistry
- PubMed
- 34259702 Full text @ Food Funct
Citation
Li, P., Zhang, M., Xie, D., Zhang, X., Zhang, S., Gao, F., Wang, Y., Hsiao, C.D., Li, X., Liu, K. (2021) Characterization and bioactivities of phospholipids from squid viscera and gonads using ultra-performance liquid chromatography-Q-exactive orbitrap/mass spectrometry-based lipidomics and zebrafish models. Food & function. 12(17):7986-7996.
Abstract
There has been great interest in phospholipids (PLs) from marine by-products due to their long-chain polyunsaturated fatty acids with unique health and functional properties. Here, marine PLs from squid viscera and gonads were comprehensively characterized and compared by UPLC-Q-Exactive Orbitrap/MS-based lipidomics analysis. A total of thirteen phospholipid classes including 1223 molecular species were identified and quantified in both resources. PC, PE and SM were further isolated from the total PLs of squid viscera and gonads, respectively. All isolated squid PL components were first evaluated for anti-inflammatory, antioxidant and cardiovascular effects using in vivo zebrafish models. Our results showed the diversity, content and physiological functions of PLs from squid by-products, which provided a basis for their future application in the nutritional and pharmaceutical industry.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping