PUBLICATION

Chordacentrum mineralization is delayed in zebrafish betaglycan-null mutants

Authors
Molina-Villa, T., Ramírez-Vidal, L., Mendoza, V., Escalante-Alcalde, D., López-Casillas, F.
ID
ZDB-PUB-210707-9
Date
2021
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   251(1): 213-225 (Journal)
Registered Authors
Lopez-Casillas, Fernando, Mendoza, Valentin, Ramirez-Vidal, Lizbeth
Keywords
none
MeSH Terms
  • Proteoglycans/genetics
  • Mice
  • Zebrafish*/genetics
  • Zebrafish*/metabolism
  • Animals
  • Receptors, Transforming Growth Factor beta*/genetics
  • Receptors, Transforming Growth Factor beta*/metabolism
  • Signal Transduction/genetics
  • Transforming Growth Factor beta/genetics
  • Transforming Growth Factor beta/metabolism
(all 10)
PubMed
34228380 Full text @ Dev. Dyn.
Abstract
The Transforming Growth Factor β (TGFβ) family is a group of related proteins that signal through a type I and type II receptors. Betaglycan, also known as the type III receptor (Tgfbr3), is a co-receptor for various ligands of the TGFβ family that participates in heart, liver and kidney development as revealed by the tgfbr3-null mouse, as well as in angiogenesis as revealed by Tgfbr3 downregulation in morphant zebrafish.
Here we present CRISPR/Cas9-derived zebrafish Tgfbr3-null mutants, which exhibited unaltered embryonic angiogenesis and developed into fertile adults. One reproducible phenotype displayed by these Tgfbr3-null mutants is delayed chordacentra mineralization, which nonetheless does not result in vertebral abnormalities in the adult fishes. We also report that the canonical TGFβ signaling pathway is needed for proper chordacentra mineralization and that Tgfbr3 absence decreases this signal in the notochordal cells responsible for this process.
Betaglycan's "ligand presentation" function contributes to the optimal TGFβ signaling required for zebrafish chordacentra mineralization. This article is protected by copyright. All rights reserved.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
una201
    Small Deletion
    una202
      Small Deletion
      una203
        Indel
        y1TgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          tgfbr3CRISPR3-tgfbr3CRISPR
          tgfbr3MO2-tgfbr3MRPHLNO
          1 - 2 of 2
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          Fish
          Antibodies
          Name Type Antigen Genes Isotypes Host Organism
          Ab1-tgfbr3Rabbit
          1 - 1 of 1
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          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          1 - 1 of 1
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          Mapping
          No data available