PUBLICATION
A hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development
- Authors
- Wang, Z., Ding, Y., Satta, S., Roustaei, M., Fei, P., Hsiai, T.K.
- ID
- ZDB-PUB-210707-10
- Date
- 2021
- Source
- PLoS Computational Biology 17: e1009175 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Blood Flow Velocity/physiology*
- Computational Biology
- Embryo, Nonmammalian/diagnostic imaging
- Embryo, Nonmammalian/physiology
- Heart*/diagnostic imaging
- Heart*/growth & development
- Heart*/physiology
- Image Processing, Computer-Assisted
- Microscopy, Fluorescence
- Myocardial Contraction/physiology
- Myocardium/metabolism
- Zebrafish/growth & development
- Zebrafish/physiology
- PubMed
- 34228702 Full text @ PLoS Comput. Biol.
Citation
Wang, Z., Ding, Y., Satta, S., Roustaei, M., Fei, P., Hsiai, T.K. (2021) A hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development. PLoS Computational Biology. 17:e1009175.
Abstract
Biomechanical forces intimately contribute to cardiac morphogenesis. However, volumetric imaging to investigate the cardiac mechanics with high temporal and spatial resolution remains an imaging challenge. We hereby integrated light-field microscopy (LFM) with light-sheet fluorescence microscopy (LSFM), coupled with a retrospective gating method, to simultaneously access myocardial contraction and intracardiac blood flow at 200 volumes per second. While LSFM allows for the reconstruction of the myocardial function, LFM enables instantaneous acquisition of the intracardiac blood cells traversing across the valves. We further adopted deformable image registration to quantify the ventricular wall displacement and particle tracking velocimetry to monitor intracardiac blood flow. The integration of LFM and LSFM enabled the time-dependent tracking of the individual blood cells and the differential rates of segmental wall displacement during a cardiac cycle. Taken together, we demonstrated a hybrid system, coupled with our image analysis pipeline, to simultaneously capture the myocardial wall motion with intracardiac blood flow during cardiac development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping