PUBLICATION

Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary

Authors
Godoi, F.G.A., Forner-Piquer, I., Randazzo, B., Habibi, H.R., Lo Nostro, F.L., Moreira, R.G., Carnevali, O.
ID
ZDB-PUB-210702-8
Date
2021
Source
Frontiers in endocrinology   12: 677853 (Journal)
Registered Authors
Carnevali, Oliana
Keywords
Danio rerio, Reproductive biomarkers, aquatic toxicology, endocrine disruption, follicular atresia, oxidative stress, phthalate
MeSH Terms
  • Plasticizers/pharmacology*
  • Follicular Atresia/drug effects*
  • Animals
  • Ovarian Follicle/drug effects*
  • Endocrine Disruptors/pharmacology*
  • Female
  • Ovary/drug effects*
  • Phthalic Acids/pharmacology*
  • DNA Fragmentation/drug effects
  • Zebrafish
(all 10)
PubMed
34194395 Full text @ Front Endocrinol (Lausanne)
Abstract
Di-isononyl phthalate (DiNP) is a plasticizer reported to elicit hormone-like activity and disrupt metabolism and reproduction in fish and other vertebrates. In general, phthalates have been used at high concentrations beyond reported environmental levels to assess their adverse effects on fish gonadal physiology. The present study exposed adult female zebrafish to a wide range of DiNP concentrations [0.42 µg L-1 (10-9 M), 4.2 µg L-1 (10-8 M), and 42 µg L-1 (10-7 M)] for 21 days. We evaluated gene expression profiles related to apoptosis, autophagy, and oxidative stress; DNA fragmentation (TUNEL assay: terminal deoxynucleotidyl transferase dUTP nick end labeling) and caspase activity (CAS3) were also examined. Exposure to 0.42 and 4.2 µg L-1 upregulated the genes coding for tnfa and baxa, sod1, prkaa1, respectively. CAS3 immunohistochemistry revealed a higher number of positive vitellogenic oocytes in ovaries exposed to 0.42 µg L-1. Subsequently, we examined the relationship between CAS3 signaling and DNA fragmentation. Accordingly, DNA fragmentation was observed in vitellogenic follicles of fish exposed to 0.42 and 4.2 μg L-1. Our results demonstrate that follicular atresia can occur after exposure to environmental levels of DiNP for 21 days, which may adversely affect the reproductive performance of female zebrafish in a non-monotonic manner.
Genes / Markers
Figures
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Expression
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Phenotype
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Mutations / Transgenics
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Human Disease / Model
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Sequence Targeting Reagents
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Fish
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Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab10-casp3polyclonal
    		IgGRabbit
    1 - 1 of 1
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    Orthology
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    Engineered Foreign Genes
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    Citation
    Godoi, F.G.A., Forner-Piquer, I., Randazzo, B., Habibi, H.R., Lo Nostro, F.L., Moreira, R.G., Carnevali, O. (2021) Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary. Frontiers in endocrinology. 12:677853.