PUBLICATION
Effect of dark environment on intestinal flora and expression of genes related to liver metabolism in zebrafish (Danio rerio)
- Authors
- Feng, C., Sarigaiqiqige, ., Liu, W., Chen, H., Dong, W., Yang, J.
- ID
- ZDB-PUB-210629-17
- Date
- 2021
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 249: 109100 (Journal)
- Registered Authors
- Dong, Wu
- Keywords
- Dark environment, Intestinal flora, Liver, Metabolism, Zebrafish
- MeSH Terms
-
- Animals
- Bacteroidetes/genetics
- Darkness
- Female
- Firmicutes/genetics
- Gastrointestinal Microbiome/genetics*
- Gastrointestinal Microbiome/physiology
- Gene Expression Regulation
- Lipid Metabolism/genetics
- Liver/metabolism*
- Liver/physiology
- Male
- Photoperiod
- RNA, Ribosomal, 16S
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics*
- PubMed
- 34174412 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Feng, C., Sarigaiqiqige, ., Liu, W., Chen, H., Dong, W., Yang, J. (2021) Effect of dark environment on intestinal flora and expression of genes related to liver metabolism in zebrafish (Danio rerio). Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 249:109100.
Abstract
To explore the effects of dark environment on intestinal flora and expression of genes related to liver metabolism in zebrafish, a total of 60 zebrafish were fed for 21 days (24 h dark treatments or 14/10 h light/dark cycle), and the influence of dark environment on gut microbes and liver gene expression was studied using sequencing analysis of intestinal flora and liver. The results showed that the body weight of fish was significantly increased in the dark group than that in the control group (P < 0.05). Compared with the control group, dark environment treatment changed the composition of dominant flora, increased the abundance of unconventional bacteria and reduced probiotics in the intestine of zebrafish. Of these, the ratio of Bacteroidetes to Firmicutes in the intestine was reduced. The genome expression of the liver showed significant changes, and liver metabolites were also affected. Meanwhile, dark environment decreased gene expression associated with changes in blood glucose, lipid metabolism and immunization. Dark environment also caused liver steatosis as observed by histological study. This study shows that dark environment treatment has an important impact on liver metabolism and intestinal microbes in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping