PUBLICATION
In vivo imaging reveals mature Oligodendrocyte division in adult Zebrafish
- Authors
- Zou, S., Hu, B.
- ID
- ZDB-PUB-210603-46
- Date
- 2021
- Source
- Cell regeneration (London, England) 10: 16 (Journal)
- Registered Authors
- Keywords
- Asymmetric division, In vivo imaging, Mature oligodendrocyte, Remyelination, Zebrafish
- MeSH Terms
- none
- PubMed
- 34075520 Full text @ Cell Regen (Lond)
Citation
Zou, S., Hu, B. (2021) In vivo imaging reveals mature Oligodendrocyte division in adult Zebrafish. Cell regeneration (London, England). 10:16.
Abstract
Whether mature oligodendrocytes (mOLs) participate in remyelination has been disputed for several decades. Recently, some studies have shown that mOLs participate in remyelination by producing new sheaths. However, whether mOLs can produce new oligodendrocytes by asymmetric division has not been proven. Zebrafish is a perfect model to research remyelination compared to other species. In this study, optic nerve crushing did not induce local mOLs death. After optic nerve transplantation from olig2:eGFP fish to AB/WT fish, olig2+ cells from the donor settled and rewrapped axons in the recipient. After identifying these rewrapping olig2+ cells as mOLs at 3 months posttransplantation, in vivo imaging showed that olig2+ cells proliferated. Additionally, in vivo imaging of new olig2+ cell division from mOLs was also captured within the retina. Finally, fine visual function was renewed after the remyelination program was completed. In conclusion, our in vivo imaging results showed that new olig2+ cells were born from mOLs by asymmetric division in adult zebrafish, which highlights the role of mOLs in the progression of remyelination in the mammalian CNS.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping