PUBLICATION
Neuroprotective Effects and Mechanisms of Procyanidins In Vitro and In Vivo
- Authors
- Chen, J., Chen, Y., Zheng, Y., Zhao, J., Yu, H., Zhu, J., Li, D.
- ID
- ZDB-PUB-210603-18
- Date
- 2021
- Source
- Molecules 26(10): (Journal)
- Registered Authors
- Keywords
- Nrf2/ARE pathway, PC12 cells, neuroprotective, procyanidins, zebrafish
- MeSH Terms
-
- Acetylcysteine/pharmacology
- Animals
- Antioxidant Response Elements
- Antioxidants/metabolism
- Glutathione Peroxidase/metabolism
- Heme Oxygenase-1/metabolism
- Hydrogen Peroxide/pharmacology
- NAD(P)H Dehydrogenase (Quinone)/metabolism
- NF-E2 Transcription Factor/metabolism
- Neuroprotective Agents/metabolism
- Neuroprotective Agents/pharmacology
- Oxidative Stress/drug effects
- PC12 Cells
- Proanthocyanidins/metabolism*
- Proanthocyanidins/pharmacology*
- Rats
- Reactive Oxygen Species/metabolism
- Zebrafish/metabolism
- PubMed
- 34067571 Full text @ Molecules
Citation
Chen, J., Chen, Y., Zheng, Y., Zhao, J., Yu, H., Zhu, J., Li, D. (2021) Neuroprotective Effects and Mechanisms of Procyanidins In Vitro and In Vivo. Molecules. 26(10):.
Abstract
This study evaluated the neuroprotective effects and mechanisms of procyanidins (PCs). In vitro, rat pheochromocytoma cells (PC12 cells) were exposed to PCs (1, 2 or 4 μg/mL) or N-Acetyl-L-cysteine (NAC) (20 μM) for 24 h, and then incubated with 200 μM of H2O2 for 24 h. Compared with H2O2 alone, PCs significantly increased antioxidant activities (e.g., glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT)), decreased levels of reactive oxygen species (ROS) and malondialdehyde (MDA), and increased nuclear factor-erythroid 2-related factor 2 (Nrf2) accumulation and increased the expression of quinone oxidoreductase 1 (NQO1), heme oxygenase 1 (HO-1), glutamate-cysteine ligase modifier subunit (GCLM), and glutamate-cysteine ligase catalytic subunit (GCLC). In vivo, zebrafish larvae (AB strain) 3 days post-fertilization (dpf) were exposed to NAC (30 μM) or PCs (4, 8 or 16 μg/mL) in the absence or presence of 300 μM of H2O2 for 4 days. Compared with H2O2 alone, PCs enhanced antioxidant activities (e.g., GSH-Px, CAT, and SOD), decreased levels of ROS and MDA, and enhanced Nrf2/ antioxidant response element (ARE) activation and raised expression levels of NQO1, HO-1, GCLM, and GCLC. In conclusion, these results indicated that PCs exerted neuroprotective effects via activating the Nrf2/ARE pathway and alleviating oxidative damage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping