PUBLICATION

Di-2-ethylhexyl phthalate induces heart looping disorders during zebrafish development

Authors
Sun, Y., Yang, F., Liu, Y., Yu, M., Wu, F., Wang, G.
ID
ZDB-PUB-210529-10
Date
2021
Source
Toxicology and industrial health   37(7): 391-397 (Journal)
Registered Authors
Keywords
Di-2-ethylhexyl phthalate, embryonic development, heart looping disorders, zebrafish
MeSH Terms
  • Animals
  • Plasticizers/toxicity
  • Zebrafish/growth & development*
  • Organogenesis/drug effects
  • Zebrafish Proteins/drug effects*
  • Membrane Glycoproteins/drug effects*
  • Embryonic Development/drug effects
  • Embryo, Nonmammalian/drug effects*
  • Occupational Exposure/adverse effects
  • Diethylhexyl Phthalate/toxicity*
  • Humans
  • Basic Helix-Loop-Helix Transcription Factors/drug effects*
  • Water Pollutants, Chemical/toxicity
  • Heart/drug effects*
  • Heart/growth & development*
(all 15)
PubMed
34047658 Full text @ Toxicol. Ind. Health
Abstract
Di-2-ethylhexyl phthalate (DEHP) is a type of plasticizer widely used in industry. It is well-known for its toxic effects to endocrine and reproductive systems and has been detected in amniotic fluid and placenta. In the present study, we explored the effects of DEHP on heart development by using zebrafish as a model organism. DEHP (0.02 pg) was injected into the yolk sac of zebrafish embryos at the one-cell stage. No significant difference was found in embryonic lethality between control and DEHP groups at 1-day postfertilization (dpf), but mortality significantly increased in DEHP groups at 2 and 3 dpf. The average heart rate was significantly reduced in the surviving DEHP-treated zebrafish larvae at 3 and 4 dpf. In addition, massive pericardial edema was found in DEHP-treated zebrafish (12.6 ± 1.5%), which was significantly higher than that of the control group. Serious heart looping disorder was also observed in DEHP-treated larvae, mainly manifested with an elongated atrial-ventricular distance. Moreover, the expression of heart development transcription factors was affected by DEHP injection. Real-time polymerase chain reaction confirmed that five transcription factors (hand2, tp53, mef2c, esr1, and tbx18) were significantly downregulated in the DEHP group at 2 dpf, and three transcription factors (zic3, tcf21, and gata4) were significantly upregulated. Our results emphasize the need for the development of a nontoxic plasticizer to prevent possible deleterious effects on humans and other life-forms.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
ABchemical treatment by environment: bis(2-ethylhexyl) phthalateLong-pec
ABchemical treatment by environment: bis(2-ethylhexyl) phthalateLong-pec
ABchemical treatment by environment: bis(2-ethylhexyl) phthalateLong-pec
ABchemical treatment by environment: bis(2-ethylhexyl) phthalateLong-pec
ABchemical treatment by environment: bis(2-ethylhexyl) phthalateLong-pec
ABchemical treatment by environment: bis(2-ethylhexyl) phthalateLong-pec
ABchemical treatment by environment: bis(2-ethylhexyl) phthalateLong-pec
ABchemical treatment by environment: bis(2-ethylhexyl) phthalateLong-pec
ABchemical treatment by environment: bis(2-ethylhexyl) phthalateLong-pec to Pec-fin
ABchemical treatment by environment: bis(2-ethylhexyl) phthalateProtruding-mouth
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Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Fish
Fish
AB
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Antibodies
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Orthology
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Citation
Sun, Y., Yang, F., Liu, Y., Yu, M., Wu, F., Wang, G. (2021) Di-2-ethylhexyl phthalate induces heart looping disorders during zebrafish development. Toxicology and industrial health. 37(7):391-397.