PUBLICATION
Vinegar-processed Curcuma phaeocaulis promotes anti-angiogenic activity and reduces toxicity in zebrafish and rat models
- Authors
- Liao, W., Chen, Y., Zhu, Z., Chen, J., Gao, T., Limsila, B., Techadamrongsin, Y., Wang, L., Yu, J., Fu, C., Li, R.
- ID
- ZDB-PUB-210525-10
- Date
- 2021
- Source
- Pharmaceutical biology 59: 410-417 (Journal)
- Registered Authors
- Keywords
- Ezhu, LC50 values, Volatile oil, cardiotoxicity, intersegmental blood vessels, ovarian coefficient, uterine coefficient
- MeSH Terms
-
- Acetic Acid/therapeutic use*
- Angiogenesis Inhibitors/isolation & purification
- Angiogenesis Inhibitors/therapeutic use*
- Animals
- Animals, Genetically Modified
- Curcuma*
- Dose-Response Relationship, Drug
- Female
- Male
- Neovascularization, Pathologic/drug therapy*
- Neovascularization, Pathologic/genetics
- Neovascularization, Pathologic/pathology
- Oils, Volatile/isolation & purification
- Oils, Volatile/therapeutic use*
- Plant Extracts/isolation & purification
- Plant Extracts/therapeutic use*
- Rats
- Rats, Sprague-Dawley
- Zebrafish
- PubMed
- 34027806 Full text @ Pharm Biol
Citation
Liao, W., Chen, Y., Zhu, Z., Chen, J., Gao, T., Limsila, B., Techadamrongsin, Y., Wang, L., Yu, J., Fu, C., Li, R. (2021) Vinegar-processed Curcuma phaeocaulis promotes anti-angiogenic activity and reduces toxicity in zebrafish and rat models. Pharmaceutical biology. 59:410-417.
Abstract
Context Processing with vinegar could enhance the efficacy and reduce the toxicity of Curcuma phaeocaulis Valeton. (Zingiberaceae), a Chinese herbal medicine with anti-inflammatory and antitumor activities.
Objective This study investigated the vinegar processing effects by evaluating anti-angiogenic effect and toxicity of C. phaeocaulis through zebrafish and rat models.
Materials and methods Zebrafish embryos (AB and FLk-GFP strain) were applied to evaluate toxicity, cardiotoxicity and anti-angiogenic activity of volatile oil, and water decoction of the raw and vinegar-processed C. phaeocaulis. Meanwhile, a blood stasis syndrome rat model was applied to study the toxicity by measuring the ovarian and uterine coefficient.
Results Curcuma phaeocaulis volatile oil and its vinegar-processed products in zebrafish had an LC50 of 67.315 and 95.755 μg/mL, respectively. Curcuma phaeocaulis water decoction and its vinegar-processed products had an LC50 of 161.440 and 206.239 μg/mL, respectively. The toxicity of vinegar-processed products was significantly lower than the raw, and the development characteristic of zebrafish embryos at different times confirmed these results. The volatile oil of vinegar-processed products could inhibit the growth of intersegmental blood vessels at the dose of 20 μg/mL, while the raw materials did not exhibit such effect at the same concentration. The rat experiment also confirmed that the volatile oil could reduce toxicity of ovarian and uterine.
Discussion and conclusions The study indicated that processing using vinegar could decrease toxicity and increase anti-angiogenic activity of C. phaeocaulis, which could be applied for clinical treatment. Further in-depth study on the synergism and detoxification mechanism of vinegar processing technology is needed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping