PUBLICATION
Myomixer is expressed during embryonic and post-larval hyperplasia, muscle regeneration and differentiation of myoblats in rainbow trout (Oncorhynchus mykiss)
- Authors
- Perello-Amoros, M., Rallière, C., Gutiérrez, J., Gabillard, J.C.
- ID
- ZDB-PUB-210508-5
- Date
- 2021
- Source
- Gene 790: 145688 (Journal)
- Registered Authors
- Gabillard, Jean-Charles
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Hyperplasia/metabolism
- Hyperplasia/pathology*
- Larva/cytology*
- Larva/metabolism
- Membrane Proteins/genetics
- Membrane Proteins/metabolism
- Muscle Development*
- Muscle Proteins/genetics
- Muscle Proteins/metabolism*
- Muscle, Skeletal
- Myoblasts/cytology*
- Myoblasts/metabolism
- Oncorhynchus mykiss/embryology*
- Oncorhynchus mykiss/metabolism
- Phylogeny
- Regeneration*
- Sequence Homology
- PubMed
- 33961974 Full text @ Gene
Citation
Perello-Amoros, M., Rallière, C., Gutiérrez, J., Gabillard, J.C. (2021) Myomixer is expressed during embryonic and post-larval hyperplasia, muscle regeneration and differentiation of myoblats in rainbow trout (Oncorhynchus mykiss). Gene. 790:145688.
Abstract
In contrast to mice or zebrafish, trout exhibits post-larval muscle growth through hypertrophy and formation of new myofibers (hyperplasia). The muscle fibers are formed by the fusion of mononucleated cells (myoblasts) regulated by several muscle-specific proteins such as Myomaker or Myomixer. In this work, we identified a unique gene encoding a Myomixer protein of 77 amino acids (aa) in the trout genome. Sequence analysis and phylogenetic tree showed moderate conservation of the overall protein sequence across teleost fish (61% of aa identity between trout and zebrafish Myomixer sequences). Nevertheless, the functionally essential motif, AxLyCxL is perfectly conserved in all studied sequences of vertebrates. Using in situ hybridization, we observed that myomixer was highly expressed in the embryonic myotome, particularly in the hyperplasic area. Moreover, myomixer remained readily expressed in white muscle of juvenile (1 and 20 g) although its expression decreased in mature fish. We also showed that myomixer is up-regulated during muscle regeneration and in vitro myoblasts differentiation. Together, these data indicate that myomixer expression is consistently associated with the formation of new myofibers during somitogenesis, post-larval growth and muscle regeneration in trout.
Errata / Notes
This article is corrected by ZDB-PUB-220906-249 .
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping