PUBLICATION

In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration

Authors
Pronobis, M.I., Zheng, S., Singh, S.P., Goldman, J.A., Poss, K.D.
ID
ZDB-PUB-210326-9
Date
2021
Source
eLIFE   10: (Journal)
Registered Authors
Goldman, Joseph, Poss, Kenneth D., Pronobis, Mira, Singh, Sumeet Pal
Keywords
developmental biology, zebrafish
Datasets
GEO:GSE168371
MeSH Terms
  • Signal Transduction
  • Heart/physiology*
  • Animals
  • Zebrafish Proteins/metabolism*
  • Zebrafish
  • Animals, Genetically Modified
  • Myocytes, Cardiac/metabolism*
  • Regeneration*/genetics
(all 8)
PubMed
33764296 Full text @ Elife
Abstract
Strategies have not been available until recently to uncover interacting protein networks specific to key cell types, their subcellular compartments, and their major regulators during complex in vivo events. Here we apply BioID2 proximity labeling to capture protein networks acting within cardiomyocytes during a key model of innate heart regeneration in zebrafish. Transgenic zebrafish expressing a promiscuous BirA2 localized to the entire myocardial cell or membrane compartment were generated, each identifying distinct proteomes in adult cardiomyocytes that became altered during regeneration. BioID2 profiling for interactors with ErbB2, a co-receptor for the cardiomyocyte mitogen Nrg1, implicated Rho A as a target of ErbB2 signaling in cardiomyocytes. Blockade of Rho A during heart regeneration, or during cardiogenic stimulation by the mitogenic influences Nrg1, Vegfaa or Vitamin D, disrupted muscle creation. Our findings reveal proximity labeling as a useful resource to interrogate cell proteomes and signaling networks during tissue regeneration in zebrafish.
Genes / Markers
Figures
Figure Gallery (15 images) / 2
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
pd10TgTransgenic Insertion
    pd36TgTransgenic Insertion
      pd68TgTransgenic Insertion
        pd69TgTransgenic Insertion
          pd70TgTransgenic Insertion
            pd107TgTransgenic Insertion
              pd262TgTransgenic Insertion
                pd335Point Mutation
                pd336TgTransgenic Insertion
                  pd337TgTransgenic Insertion
                    1 - 10 of 10
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                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    No data available
                    Fish
                    Fish
                    pd68Tg
                    pd69Tg
                    pd70Tg
                    1 - 3 of 3
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                    Antibodies
                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    BFPEFGBFP
                    BioID2EFGBioID2
                    CreEFGCre
                    DipToxEFGDipTox
                    DsRedEFGDsRed
                    GFPEFGGFP
                    mCherryEFGmCherry
                    TagBFPEFGTagBFP
                    1 - 8 of 8
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                    Mapping
                    No data available
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                    Citation
                    Pronobis, M.I., Zheng, S., Singh, S.P., Goldman, J.A., Poss, K.D. (2021) In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration. eLIFE. 10:.