PUBLICATION
Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion
- Authors
- Faria, M., Prats, E., Rosas Ramírez, J.R., Bellot, M., Bedrossiantz, J., Pagano, M., Valls, A., Gomez-Canela, C., Porta, J.M., Mestres, J., Garcia-Reyero, N., Faggio, C., Gómez Oliván, L.M., Raldua, D.
- ID
- ZDB-PUB-210224-9
- Date
- 2021
- Source
- The Science of the total environment 775: 145671 (Journal)
- Registered Authors
- Keywords
- Acetylcholinesterase inhibitor, Androgenic effects, Endocrine disruptors, Neurotoxicity, Predicted target profile, Risk assessment
- MeSH Terms
-
- Androgens
- Ecosystem
- Insecticides*/toxicity
- Fenitrothion*/toxicity
- Larva
- PubMed
- 33621872 Full text @ Sci. Total Environ.
Abstract
Fenitrothion is an organophosphorus insecticide usually found in aquatic ecosystems at concentrations in the range of low ng/L. In this manuscript we show that 24 h exposure to environmental concentrations of fenitrothion, from ng/L to low μg/L, altered basal locomotor activity, visual-motor response and acoustic/vibrational escape response of zebrafish larvae. Furthermore, fenitrothion and expression of gap43a, gfap, atp2b1a, and mbp exhibited a significant non-monotonic concentration-response relationship. Once determined that environmental concentrations of fenitrothion were neurotoxic for zebrafish larvae, a computational analysis identified potential protein targets of this compound. Some of the predictions, including interactions with acetylcholinesterase, monoamine-oxidases and androgen receptor (AR), were experimentally validated. Binding to AR was the most suitable candidate for molecular initiating event, as indicated by both the up-regulation of cyp19a1b and sult2st3 and the non-monotonic relationship found between fenitrothion and the observed responses. Finally, when the integrity of the monoaminergic system was evaluated, altered levels of L-DOPA, DOPAC, HVA and 5-HIAA were found, as well as a significant up-regulation of slc18a2 expression at the lowest concentrations of fenitrothion. These data strongly suggest that concentrations of fenitrothion commonly found in aquatic ecosystems present a significant environmental risk for fish communities.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping