PUBLICATION

DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish

Authors
Cuomo, M., Borrelli, L., Della Monica, R., Coretti, L., De Riso, G., D'Angelo Lancellotti di Durazzo, L., Fioretti, A., Lembo, F., Dinan, T.G., Cryan, J.F., Cocozza, S., Chiariotti, L.
ID
ZDB-PUB-210128-17
Date
2021
Source
Biomolecules   11(2): (Journal)
Registered Authors
Keywords
DNA methylation, Zebrafish, cell-to-cell heterogeneity, epialleles, methylation profiles, microbiota?gut?brain axis
MeSH Terms
  • Brain/metabolism
  • Promoter Regions, Genetic
  • Brain-Derived Neurotrophic Factor/genetics*
  • Brain-Derived Neurotrophic Factor/metabolism
  • Female
  • Gene Expression Regulation
  • Microbiota
  • Epigenesis, Genetic
  • Gastrointestinal Microbiome
  • Gene Expression Profiling
  • Behavior, Animal
  • Computational Biology/methods
  • CpG Islands
  • Probiotics
  • Tryptophan Hydroxylase/genetics*
  • Tryptophan Hydroxylase/metabolism
  • Zebrafish
  • Animals
  • Alleles
  • Gene Library
  • Lacticaseibacillus rhamnosus
  • DNA Methylation*
  • Male
(all 23)
PubMed
33499115 Full text @ Biomolecules
Abstract
The bidirectional microbiota-gut-brain axis has raised increasing interest over the past years in the context of health and disease, but there is a lack of information on molecular mechanisms underlying this connection. We hypothesized that change in microbiota composition may affect brain epigenetics leading to long-lasting effects on specific brain gene regulation. To test this hypothesis, we used Zebrafish (Danio Rerio) as a model system. As previously shown, treatment with high doses of probiotics can modulate behavior in Zebrafish, causing significant changes in the expression of some brain-relevant genes, such as BDNF and Tph1A. Using an ultra-deep targeted analysis, we investigated the methylation state of the BDNF and Tph1A promoter region in the brain and gut of probiotic-treated and untreated Zebrafishes. Thanks to the high resolution power of our analysis, we evaluated cell-to-cell methylation differences. At this resolution level, we found slight DNA methylation changes in probiotic-treated samples, likely related to a subgroup of brain and gut cells, and that specific DNA methylation signatures significantly correlated with specific behavioral scores.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
No data available
Antibodies
No data available
Orthology
No data available
Engineered Foreign Genes
No data available
Mapping
No data available
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Citation
Cuomo, M., Borrelli, L., Della Monica, R., Coretti, L., De Riso, G., D'Angelo Lancellotti di Durazzo, L., Fioretti, A., Lembo, F., Dinan, T.G., Cryan, J.F., Cocozza, S., Chiariotti, L. (2021) DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish. Biomolecules. 11(2):.