ZFIN ID: ZDB-PUB-210101-6
Calcineurin controls proximodistal blastema polarity in zebrafish fin regeneration
Cao, Z., Meng, Y., Gong, F., Xu, Z., Liu, F., Fang, M., Zou, L., Liao, X., Wang, X., Luo, L., Li, X., Lu, H.
Date: 2021
Source: Proceedings of the National Academy of Sciences of the United States of America   118(2): (Journal)
Registered Authors: Lu, Huiqiang, Luo, Lingfei
Keywords: calcineurin, fin regeneration, polarity, zebrafish
Microarrays: GEO:GSE159560
MeSH Terms:
  • Animal Fins/physiology*
  • Animals
  • Calcineurin/metabolism*
  • Cell Polarity/physiology
  • Extremities/physiology
  • Regeneration/physiology*
  • Signal Transduction
  • Wound Healing/physiology
  • Zebrafish/metabolism
  • Zebrafish Proteins
PubMed: 33376206 Full text @ Proc. Natl. Acad. Sci. USA
Planarian flatworms regenerate their heads and tails from anterior or posterior wounds and this regenerative blastema polarity is controlled by Wnt/β-catenin signaling. It is well known that a regeneration blastema of appendages of vertebrates such as fish and amphibians grows distally. However, it remains unclear whether a regeneration blastema in vertebrate appendages can grow proximally. Here, we show that a regeneration blastema in zebrafish fins can grow proximally along the proximodistal axis by calcineurin inhibition. We used fin excavation in adult zebrafish to observe unidirectional regeneration from the anterior cut edge (ACE) to the posterior cut edge (PCE) of the cavity and this unidirectional regeneration polarity occurs as the PCE fails to build blastemas. Furthermore, we found that calcineurin activities in the ACE were greater than in the PCE. Calcineurin inhibition induced PCE blastemas, and calcineurin hyperactivation suppressed fin regeneration. Collectively, these findings identify calcineurin as a molecular switch to specify the PCE blastema of the proximodistal axis and regeneration polarity in zebrafish fin.