PUBLICATION

Emx2 regulates hair cell rearrangement but not positional identity within neuromasts

Authors
Ohta, S., Ji, Y.R., Martin, D., Wu, D.K.
ID
ZDB-PUB-210101-10
Date
2020
Source
eLIFE   9: (Journal)
Registered Authors
Keywords
developmental biology, zebrafish
Datasets
GEO:GSE152859
MeSH Terms
  • Cell Polarity/physiology
  • Gene Expression Regulation, Developmental/physiology*
  • Lateral Line System/physiology
  • Zebrafish/metabolism
  • Hair Cells, Auditory/metabolism*
  • Animals
  • Transcription Factors/metabolism*
  • Homeodomain Proteins/metabolism*
(all 8)
PubMed
33377867 Full text @ Elife
Abstract
Each hair cell (HC) precursor of zebrafish neuromasts divides to form two daughter HCs of opposite hair bundle orientations. Previously, we showed that transcription factor Emx2, expressed in only one of the daughter HCs, generates this bidirectional HC pattern (Jiang et al., 2017). Here, we asked whether Emx2 mediates this effect by changing location of hair bundle establishment or positions of HCs since daughter HCs are known to switch positions with each other. We showed this HC rearrangement, redefined as two processes named Rock & Roll, is required for positional acquisition of HCs. Apical protrusion formation of nascent HCs and planar polarity signaling are both important for the Rock & Roll. Emx2 facilitates Rock & Roll by delaying apical protrusion of its nascent HCs but it does not determine HCs' ultimate positions, indicating that Emx2 mediates bidirectional HC pattern by changing the location where hair bundle is established in HCs.
Genes / Markers
Figures
Figure Gallery (15 images) / 2
Show all Figures
Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
atoh1aWTstandard conditionsDay 4RNA-Seq
emx2Ab1-emx2vo8Tgstandard conditionsDay 4IHC
emx2Ab1-emx2vo8Tgstandard conditionsDay 4IHC
myo6bWTstandard conditionsDay 4RNA-Seq
pvalb8WTstandard conditionsDay 4RNA-Seq
s100sWTstandard conditionsDay 4RNA-Seq
s100tWTstandard conditionsDay 4RNA-Seq
tekt3WTstandard conditionsDay 4RNA-Seq
1 - 8 of 8
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Phenotype
Fish Conditions Stage Phenotype Figure
emx2idc5/idc5; vo8Tgstandard conditionsLong-pec
emx2idc5/idc5; vo8Tgstandard conditionsLong-pec
idc16Tg; zf3465Tgchemical treatment by environment: nocodazoleLong-pec
vangl2m209/m209; vo8Tg; zf3465Tgstandard conditionsLong-pec
1 - 4 of 4
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
idc4TgTransgenic Insertion
    idc5
      		Small Deletion
      idc10TgTransgenic Insertion
        idc16TgTransgenic Insertion
          m209
            		Point Mutation
            vo8TgTransgenic Insertion
              vo13TgTransgenic Insertion
                zf3465TgTransgenic Insertion
                  1 - 8 of 8
                  Show
                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  Target Reagent Reagent Type
                  emx2CRISPR7-emx2CRISPR
                  1 - 1 of 1
                  Show
                  Fish
                  Fish
                  emx2idc5/idc5; vo8Tg
                  idc16Tg; zf3465Tg
                  vangl2m209/m209; vo8Tg; zf3465Tg
                  vo8Tg
                  WT
                  1 - 5 of 5
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                  Antibodies
                  Name Type Antigen Genes Isotypes Host Organism
                  Ab1-emx2polyclonalRabbit
                  1 - 1 of 1
                  Show
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  EGFPEFGEGFP
                  GFPEFGGFP
                  mCherryEFGmCherry
                  TomatoEFGTomato
                  1 - 4 of 4
                  Show
                  Mapping
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                  Citation
                  Ohta, S., Ji, Y.R., Martin, D., Wu, D.K. (2020) Emx2 regulates hair cell rearrangement but not positional identity within neuromasts. eLIFE. 9:.