PUBLICATION

Coordinated assembly and release of adhesions builds apical junctional belts during de novo polarisation of an epithelial tube

Authors

Symonds, A.C., Buckley, C.E., Williams, C.A., Clarke, J.D.W.

ID

ZDB-PUB-201229-32

Date

2020

Source

Development (Cambridge, England) 147(24): (Journal)

Registered Authors

Clarke, Jon

Keywords

Adhesions, Epithelial tube, Morphogenesis, Neural tube, Zebrafish

MeSH Terms

Epithelial Cells/metabolism
Neural Tube/growth & development
Animals
Morphogenesis/genetics*
rab GTP-Binding Proteins/genetics*
Gene Expression Regulation, Developmental/genetics
Membrane Proteins
Guanylate Cyclase/genetics*
Cell Polarity/genetics
Zebrafish Proteins/genetics*
Zebrafish/genetics
Zebrafish/growth & development*
Intercellular Junctions/genetics

PubMed

33361092 Full text @ Development

Abstract

Using the zebrafish neural tube as a model, we uncover the in vivo mechanisms allowing the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised, solid organ. We show that Mpp5a and Rab11a play a dual role in coordinating the generation of ipsilateral junctional belts whilst simultaneously releasing contralateral adhesions across the centre of the tissue. We show that Mpp5a- and Rab11a-mediated resolution of cell-cell adhesions are both necessary for midline lumen opening and contribute to later maintenance of epithelial organisation. We propose that these roles for both Mpp5a and Rab11a operate through the transmembrane protein Crumbs. In light of a recent conflicting publication, we also clarify that the junction-remodelling role of Mpp5a is not specific to dividing cells.

Genes / Markers

Figures