PUBLICATION
Pharyngeal pouches provide a niche microenvironment for arch artery progenitor specification
- Authors
- Mao, A., Zhang, M., Li, L., Liu, J., Ning, G., Cao, Y., Wang, Q.
- ID
- ZDB-PUB-201219-4
- Date
- 2020
- Source
- Development (Cambridge, England) 148(2): (Journal)
- Registered Authors
- Wang, Qiang
- Keywords
- BMP signal, Niche microenvironment, Pharyngeal arch artery morphogenesis, Pharyngeal pouch, Progenitor specification
- MeSH Terms
-
- Animals
- Arteries/embryology*
- Body Patterning*/genetics
- Bone Morphogenetic Proteins/metabolism
- Branchial Region/blood supply*
- Cell Differentiation
- Cell Lineage/genetics
- Cell Movement
- Cellular Microenvironment*
- Endothelial Cells/cytology
- Gene Expression Regulation, Developmental
- Mesoderm/embryology
- Morphogenesis
- Signal Transduction
- Stem Cells/cytology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 33334861 Full text @ Development
Citation
Mao, A., Zhang, M., Li, L., Liu, J., Ning, G., Cao, Y., Wang, Q. (2020) Pharyngeal pouches provide a niche microenvironment for arch artery progenitor specification. Development (Cambridge, England). 148(2):.
Abstract
The paired pharyngeal arch arteries (PAAs) are transient blood vessels connecting the heart with the dorsal aorta during embryogenesis. Although PAA malformations often occur along with pharyngeal pouch defects, the functional interaction between these adjacent tissues remains largely unclear. Here we report that pharyngeal pouches are essential for PAA progenitor specification in zebrafish embryos. We reveal that the segmentation of pharyngeal pouches coincides spatiotemporally with the emergence of PAA progenitor clusters. These pouches physically associate with pharyngeal mesoderm in discrete regions and provide a niche microenvironment for PAA progenitor commitment by expressing BMP proteins. Specifically, pouch-derived BMP2a and BMP5 are the primary niche cues responsible for activating the BMP/Smad pathway in pharyngeal mesoderm, thereby promoting progenitor specification. In addition, BMP2a and BMP5 play an inductive function in the expression of the cloche gene npas4l in PAA progenitors. cloche mutants exhibit a striking failure to specify PAA progenitors and display ectopic expression of head muscle markers in the pharyngeal mesoderm. Therefore, our results support a critical role of pharyngeal pouches in establishing a progenitor niche for PAA morphogenesis via BMP2a/5 expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping