PUBLICATION

Crocins from Crocus sativus L. in the Management of Hyperglycemia. In Vivo Evidence from Zebrafish

Authors
Kakouri, E., Agalou, A., Kanakis, C., Beis, D., Tarantilis, P.A.
ID
ZDB-PUB-201120-108
Date
2020
Source
Molecules   25(22): (Journal)
Registered Authors
Beis, Dimitris
Keywords
crocins, glucose, insulin, pck1, ?-pancreatic cells
MeSH Terms
  • Phosphoenolpyruvate Carboxykinase (GTP)/metabolism
  • Insulin-Secreting Cells/metabolism
  • Blood Glucose/metabolism
  • Gluconeogenesis
  • Animals
  • Insulin/metabolism
  • Zebrafish
  • Crocus/chemistry*
  • Carotenoids/analysis
  • Carotenoids/pharmacology*
  • Glucose/metabolism
  • Hyperglycemia/drug therapy*
  • Animals, Genetically Modified
  • Pancreas/embryology
  • Pancreas/metabolism
  • Plant Extracts/pharmacology
  • Ions
(all 17)
PubMed
33182581 Full text @ Molecules
Abstract
Diabetes mellitus is a disease characterized by persistent high blood glucose levels and accompanied by impaired metabolic pathways. In this study, we used zebrafish to investigate the effect of crocins isolated from Crocus sativus L., on the control of glucose levels and pancreatic β-cells. Embryos were exposed to an aqueous solution of crocins and whole embryo glucose levels were measured at 48 h post-treatment. We showed that the application of crocins reduces zebrafish embryo glucose levels and enhances insulin expression. We also examined whether crocins are implicated in the metabolic pathway of gluconeogenesis. We showed that following a single application of crocins and glucose level reduction, the expression of phosphoenolpyruvate carboxykinase1 (pck1), a key gene involved in glucose metabolism, is increased. We propose a putative role for the crocins in glucose metabolism and insulin management.
Genes / Markers
Figures
Figure Gallery (1 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
m1018Tgchemical treatment by environment: crocin-1Day 5IFL
m1018Tgchemical treatment by environment: crocin-1Day 5RTPCR
WTchemical treatment by environment: crocin-1Day 5RTPCR
1 - 3 of 3
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Phenotype
Fish Conditions Stage Phenotype Figure
WTchemical treatment by environment: crocin-1Day 5
WTchemical treatment by environment: crocin-1Day 5
m1018Tgchemical treatment by environment: crocin-1Day 5
m1018Tgchemical treatment by environment: crocin-1Day 5
1 - 4 of 4
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m1018TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    Human Disease Fish Conditions Evidence
    diabetes mellitusTAS
    1 - 1 of 1
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    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    m1018Tg
    WT
    1 - 2 of 2
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    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    DsRedEFGDsRed
    1 - 1 of 1
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    Mapping
    No data available
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    Citation
    Kakouri, E., Agalou, A., Kanakis, C., Beis, D., Tarantilis, P.A. (2020) Crocins from Crocus sativus L. in the Management of Hyperglycemia. In Vivo Evidence from Zebrafish. Molecules. 25(22):.