PUBLICATION
Dual Oxidase Mutant Retards Mauthner-Cell Axon Regeneration at an Early Stage via Modulating Mitochondrial Dynamics in Zebrafish
- Authors
- Yang, L.Q., Chen, M., Ren, D.L., Hu, B.
- ID
- ZDB-PUB-201031-19
- Date
- 2020
- Source
- Neuroscience Bulletin 36(12): 1500-1512 (Journal)
- Registered Authors
- Hu, Bing
- Keywords
- Axon regeneration, Mauthner cell, Mitochondrial dynamics, Zebrafish, duox
- Datasets
- GEO:GSE144689
- MeSH Terms
-
- Animals
- Axons/physiology*
- CRISPR-Cas Systems
- Dual Oxidases/genetics*
- Mitochondrial Dynamics*
- Nerve Regeneration*
- Transcriptome
- Zebrafish*
- PubMed
- 33123984 Full text @ Neurosci. Bull.
Citation
Yang, L.Q., Chen, M., Ren, D.L., Hu, B. (2020) Dual Oxidase Mutant Retards Mauthner-Cell Axon Regeneration at an Early Stage via Modulating Mitochondrial Dynamics in Zebrafish. Neuroscience Bulletin. 36(12):1500-1512.
Abstract
Dual oxidase (duox)-derived reactive oxygen species (ROS) have been correlated with neuronal polarity, cerebellar development, and neuroplasticity. However, there have not been many comprehensive studies of the effect of individual duox isoforms on central-axon regeneration in vivo. Here, we explored this question in zebrafish, an excellent model organism for central-axon regeneration studies. In our research, mutation of the duox gene with CRISPR/Cas9 significantly retarded the single-axon regeneration of the zebrafish Mauthner cell in vivo. Using deep transcriptome sequencing, we found that the expression levels of related functional enzymes in mitochondria were down-regulated in duox mutant fish. In vivo imaging showed that duox mutants had significantly disrupted mitochondrial transport and redox state in single Mauthner-cell axon. Our research data provide insights into how duox is involved in central-axon regeneration by changing mitochondrial transport.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping