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ZFIN ID: ZDB-PUB-201024-4
Lineage analysis reveals an endodermal contribution to the vertebrate pituitary
Fabian, P., Tseng, K.C., Smeeton, J., Lancman, J.J., Dong, P.D.S., Cerny, R., Crump, J.G.
Date: 2020
Source: Science (New York, N.Y.)   370: 463-467 (Journal)
Registered Authors: Crump, Gage DeKoeyer, Dong, P. Duc, Lancman, Joseph
Keywords: none
Microarrays: GEO:GSE148591
MeSH Terms:
  • Animals
  • Cell Lineage
  • Endoderm/cytology
  • Endoderm/embryology*
  • Pituitary Gland, Anterior/cytology
  • Pituitary Gland, Anterior/embryology*
  • RNA-Seq
  • Single-Cell Analysis
  • Zebrafish
PubMed: 33093109 Full text @ Science
Vertebrate sensory organs arise from epithelial thickenings called placodes. Along with neural crest cells, cranial placodes are considered ectodermal novelties that drove evolution of the vertebrate head. The anterior-most placode generates the endocrine lobe [adenohypophysis (ADH)] of the pituitary, a master gland controlling growth, metabolism, and reproduction. In addition to known ectodermal contributions, we use lineage tracing and time-lapse imaging in zebrafish to identify an endodermal contribution to the ADH. Single-cell RNA sequencing of the adult pituitary reveals similar competency of endodermal and ectodermal epithelia to generate all endocrine cell types. Further, endoderm can generate a rudimentary ADH-like structure in the near absence of ectodermal contributions. The fish condition supports the vertebrate pituitary arising through interactions of an ancestral endoderm-derived proto-pituitary with newly evolved placodal ectoderm.