|ZFIN ID: ZDB-PUB-201024-4|
Lineage analysis reveals an endodermal contribution to the vertebrate pituitary
Fabian, P., Tseng, K.C., Smeeton, J., Lancman, J.J., Dong, P.D.S., Cerny, R., Crump, J.G.
|Source:||Science (New York, N.Y.) 370: 463-467 (Journal)|
|Registered Authors:||Crump, Gage DeKoeyer, Dong, P. Duc, Lancman, Joseph|
|PubMed:||33093109 Full text @ Science|
Fabian, P., Tseng, K.C., Smeeton, J., Lancman, J.J., Dong, P.D.S., Cerny, R., Crump, J.G. (2020) Lineage analysis reveals an endodermal contribution to the vertebrate pituitary. Science (New York, N.Y.). 370:463-467.
ABSTRACTVertebrate sensory organs arise from epithelial thickenings called placodes. Along with neural crest cells, cranial placodes are considered ectodermal novelties that drove evolution of the vertebrate head. The anterior-most placode generates the endocrine lobe [adenohypophysis (ADH)] of the pituitary, a master gland controlling growth, metabolism, and reproduction. In addition to known ectodermal contributions, we use lineage tracing and time-lapse imaging in zebrafish to identify an endodermal contribution to the ADH. Single-cell RNA sequencing of the adult pituitary reveals similar competency of endodermal and ectodermal epithelia to generate all endocrine cell types. Further, endoderm can generate a rudimentary ADH-like structure in the near absence of ectodermal contributions. The fish condition supports the vertebrate pituitary arising through interactions of an ancestral endoderm-derived proto-pituitary with newly evolved placodal ectoderm.