PUBLICATION
Emergence of Neuronal Diversity during Vertebrate Brain Development
- Authors
- Raj, B., Farrell, J.A., Liu, J., El Kholtei, J., Carte, A.N., Navajas Acedo, J., Du, L.Y., McKenna, A., Relić, Đ., Leslie, J.M., Schier, A.F.
- ID
- ZDB-PUB-201020-52
- Date
- 2020
- Source
- Neuron 108(6): 1058-1074.e6 (Journal)
- Registered Authors
- Farrell, Jeffrey, Navajas Acedo, Joaquin, Raj, Bushra, Schier, Alexander
- Keywords
- CRISPR-Cas9 barcoding, brain, cell specification, lineage, neurogenesis, progenitor, retina, scRNA-Seq, transcriptional cascade, zebrafish
- Datasets
- GEO:GSE158142
- MeSH Terms
-
- Animals
- Brain/cytology
- Brain/growth & development*
- Cell Differentiation/physiology
- Cell Lineage/physiology*
- Gene Expression Regulation, Developmental
- Neurogenesis/physiology*
- Neurons/cytology*
- Zebrafish/genetics*
- PubMed
- 33068532 Full text @ Neuron
Citation
Raj, B., Farrell, J.A., Liu, J., El Kholtei, J., Carte, A.N., Navajas Acedo, J., Du, L.Y., McKenna, A., Relić, Đ., Leslie, J.M., Schier, A.F. (2020) Emergence of Neuronal Diversity during Vertebrate Brain Development. Neuron. 108(6):1058-1074.e6.
Abstract
Neurogenesis comprises many highly regulated processes including proliferation, differentiation, and maturation. However, the transcriptional landscapes underlying brain development are poorly characterized. We describe a developmental single-cell catalog of ∼220,000 zebrafish brain cells encompassing 12 stages from embryo to larva. We characterize known and novel gene markers for ∼800 clusters and provide an overview of the diversification of neurons and progenitors across these time points. We also introduce an optimized GESTALT lineage recorder that enables higher expression and recovery of Cas9-edited barcodes to query lineage segregation. Cell type characterization indicates that most embryonic neural progenitor states are transitory and transcriptionally distinct from neural progenitors of post-embryonic stages. Reconstruction of cell specification trajectories reveals that late-stage retinal neural progenitors transcriptionally overlap cell states observed in the embryo. The zebrafish brain development atlas provides a resource to define and manipulate specific subsets of neurons and to uncover the molecular mechanisms underlying vertebrate neurogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping