PUBLICATION
Genetically encoded thiol redox-sensors in the zebrafish model: lessons for embryonic development and regeneration
- Authors
- Breus, O., Dickmeis, T.
- ID
- ZDB-PUB-201007-8
- Date
- 2020
- Source
- Biological chemistry 402(3): 363-378 (Review)
- Registered Authors
- Breus, Oksana, Dickmeis, Thomas
- Keywords
- embryo, glutathione, hydrogen peroxide, imaging, regeneration, zebrafish
- MeSH Terms
-
- Animals
- Models, Animal
- Oxidation-Reduction
- Reactive Oxygen Species/metabolism
- Regeneration*
- Sulfhydryl Compounds/metabolism*
- Zebrafish/embryology
- PubMed
- 33021959 Full text @ Biol. Chem.
Citation
Breus, O., Dickmeis, T. (2020) Genetically encoded thiol redox-sensors in the zebrafish model: lessons for embryonic development and regeneration. Biological chemistry. 402(3):363-378.
Abstract
Important roles for reactive oxygen species (ROS) and redox signaling in embryonic development and regenerative processes are increasingly recognized. However, it is difficult to obtain information on spatiotemporal dynamics of ROS production and signaling in vivo. The zebrafish is an excellent model for in vivo bioimaging and possesses a remarkable regenerative capacity upon tissue injury. Here, we review data obtained in this model system with genetically encoded redox-sensors targeting H2O2 and glutathione redox potential. We describe how such observations have prompted insight into regulation and downstream effects of redox alterations during tissue differentiation, morphogenesis and regeneration. We also discuss the properties of the different sensors and their consequences for the interpretation of in vivo imaging results. Finally, we highlight open questions and additional research fields that may benefit from further application of such sensor systems in zebrafish models of development, regeneration and disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping