PUBLICATION

Tie1 regulates zebrafish cardiac morphogenesis through tolloid-like 1 expression

Authors
Carlantoni, C., Allanki, S., Kontarakis, Z., Rossi, A., Piesker, J., Günther, S., Stainier, D.Y.R.
ID
ZDB-PUB-201002-150
Date
2020
Source
Developmental Biology   469: 54-67 (Journal)
Registered Authors
Kontarakis, Zacharias, Stainier, Didier
Keywords
Endothelial cells, Heart, Tie1, Tolloid-like 1, Zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Endothelial Cells/cytology
  • Endothelium, Vascular/cytology
  • Extracellular Matrix Proteins/genetics
  • Extracellular Matrix Proteins/metabolism
  • Gene Expression Regulation
  • Heart/embryology*
  • Heart Defects, Congenital/genetics
  • Morphogenesis
  • Mutation
  • Myocytes, Cardiac/cytology
  • Receptor, TIE-1/genetics
  • Receptor, TIE-1/physiology
  • Tolloid-Like Metalloproteinases/genetics
  • Tolloid-Like Metalloproteinases/metabolism*
  • Transcriptome
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Zebrafish Proteins/physiology*
PubMed
32971120 Full text @ Dev. Biol.
Abstract
Tie1 is a receptor tyrosine kinase expressed in endothelial cells, where it modulates Angiopoietin/Tie2 signaling. Previous studies have shown that mouse Tie1 mutants exhibit severe cardiovascular defects; however, much remains to be learned about the role of Tie1, especially during cardiac development. To further understand Tie1 function, we generated a zebrafish tie1 mutant line. Homozygous mutant embryos display reduced endothelial and endocardial cell numbers and reduced heart size. Live imaging and ultrastructural analyses at embryonic stages revealed increased cardiac jelly thickness as well as cardiomyocyte defects, including a loss of sarcomere organization and altered cell shape. Transcriptomic profiling of embryonic hearts uncovered the downregulation of tll1, which encodes a Tolloid-like protease, in tie1-/- compared to wild-type siblings. Using mRNA injections into one-cell stage embryos, we found that tll1 overexpression could partially rescue the tie1 mutant cardiac phenotypes including the endocardial and myocardial cell numbers as well as the cardiac jelly thickness. Altogether, our results indicate the importance of a Tie1-Tolloid-like 1 axis in paracrine signaling during cardiac development.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping