PUBLICATION
Protocol for Dissection and Dissociation of Zebrafish Telencephalon for Single-Cell Sequencing
- Authors
- Cosacak, M.I., Bhattarai, P., Kizil, C.
- ID
- ZDB-PUB-200825-11
- Date
- 2020
- Source
- STAR protocols 1: 100042 (Other)
- Registered Authors
- Bhattarai, Prabesh, Cosacak, Mehmet Ilyas, Kizil, Caghan
- Keywords
- none
- MeSH Terms
-
- Humans
- Telencephalon/cytology*
- Dissection/methods*
- Zebrafish/surgery*
- Animals
- Single-Cell Analysis/methods*
- PubMed
- 32835293 Full text @ STAR Protoc
Citation
Cosacak, M.I., Bhattarai, P., Kizil, C. (2020) Protocol for Dissection and Dissociation of Zebrafish Telencephalon for Single-Cell Sequencing. STAR protocols. 1:100042.
Abstract
Single-cell sequencing (sc-Seq) is a powerful tool to investigate the molecular signatures of cell types in a complex mixture of cells. A critical step in sc-Seq is preparing a single-cell suspension with a high number of viable cells. Here, we show how to dissect zebrafish telencephalon and how to dissociate it into a single-cell suspension. This is followed by flow cytometry-based sorting to enrich for neural progenitor stem cells. Our technique typically yields 70,000 live cells from one zebrafish telencephalon. For complete details on the use and execution of this protocol, please refer to Cosacak et al. (2019).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping