PUBLICATION

Generation of foxn1/Casper Mutant Zebrafish for Allograft and Xenograft of Normal and Malignant Cells

Authors
Lv, P., Ma, D., Gao, S., Zhang, Y., Bae, Y.K., Liang, G., Gao, S., Choi, J.H., Kim, C.H., Wang, L., Liu, F.
ID
ZDB-PUB-200822-23
Date
2020
Source
Stem Cell Reports   15(3): 749-760 (Journal)
Registered Authors
Bae, Young Ki, Choi, Jung-Hwa, Gao, Shuai, Kim, Cheol-Hee, Liu, Feng, Lv, Peng, Ma, Dongyuan, Zhang, Yifan
Keywords
foxn1/Casper mutant, hematopoietic stem cells, nonconditioned cell transplantation, xenograft, zebrafish
MeSH Terms
  • Allografts/transplantation*
  • Animals
  • Base Sequence
  • Fetal Stem Cells/cytology
  • Forkhead Transcription Factors/genetics*
  • Forkhead Transcription Factors/metabolism
  • Hematopoietic Stem Cell Transplantation
  • Hematopoietic Stem Cells/cytology
  • Heterografts/transplantation*
  • Mutation/genetics*
  • Neoplasms/pathology*
  • Treatment Outcome
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed
32822590 Full text @ Stem Cell Reports
Abstract
Cell transplantation into immunodeficient recipients is a widely used approach to study stem cell and cancer biology; however, studying cell states post transplantation in vivo is inconvenient in mammals. Here, we generated a foxn1/Casper mutant zebrafish that is transparent and exhibits T cell deficiency. By employing the line for hematopoietic stem cell (HSC) transplantation (HSCT), we could achieve nonconditioned transplantation. Meanwhile, we found that fetal HSCs from 3 days post fertilization zebrafish embryos produce a better transplant outcome in foxn1/Casper mutants, compared with adult HSCs. In addition to HSCT, the foxn1/Casper mutant is feasible for allografts of myelodysplastic syndrome-like and muscle cells, as well as xenografts of medaka muscle cells. In summary, foxn1/Casper mutants permit the nonconditioned engraftment of multiple cell types and visualized characterization of transplanted cells in vivo.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes