PUBLICATION

Microtubules and motor proteins support zebrafish neuronal migration by directing cargo

Authors
Theisen, U., Ernst, A.U., Heyne, R.L.S., Ring, T.P., Thorn-Seshold, O., Köster, R.W.
ID
ZDB-PUB-200716-11
Date
2020
Source
The Journal of cell biology   219(10): (Journal)
Registered Authors
Köster, Reinhard W., Theisen, Ulrike
Keywords
none
MeSH Terms
  • Animals
  • Cadherins/genetics*
  • Cell Movement/genetics
  • Dyneins/genetics
  • Embryo, Nonmammalian
  • Embryonic Development/genetics*
  • Golgi Apparatus/genetics
  • Kinesins/genetics*
  • Microtubule Proteins/genetics
  • Microtubules/genetics
  • Molecular Motor Proteins/genetics
  • Neurons/metabolism*
  • Zebrafish/genetics
  • Zebrafish/growth & development
PubMed
32668451 Full text @ J. Cell Biol.
Abstract
Neuronal migration during development is necessary to form an ordered and functional brain. Postmitotic neurons require microtubules and dynein to move, but the mechanisms by which they contribute to migration are not fully characterized. Using tegmental hindbrain nuclei neurons in zebrafish embryos together with subcellular imaging, optogenetics, and photopharmacology, we show that, in vivo, the centrosome's position relative to the nucleus is not linked to greatest motility in this cell type. Nevertheless, microtubules, dynein, and kinesin-1 are essential for migration, and we find that interference with endosome formation or the Golgi apparatus impairs migration to a similar extent as disrupting microtubules. In addition, an imbalance in the traffic of the model cargo Cadherin-2 also reduces neuronal migration. These results lead us to propose that microtubules act as cargo carriers to control spatiotemporal protein distribution, which in turn controls motility. This adds crucial insights into the variety of ways that microtubules can support successful neuronal migration in vivo.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes