PUBLICATION

Development of the anterior lateral line system through local tissue-tissue interactions in the zebrafish head

Authors
Iwasaki, M., Yokoi, H., Suzuki, T., Kawakami, K., Wada, H.
ID
ZDB-PUB-200715-10
Date
2020
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   249(12): 1440-1454 (Journal)
Registered Authors
Kawakami, Koichi, Suzuki, Tohru, Wada, Hironori
Keywords
Rspo2, Wnt/?-catenin signaling, neural crest, neuromasts, placode
MeSH Terms
  • Intercellular Signaling Peptides and Proteins/genetics*
  • Intercellular Signaling Peptides and Proteins/metabolism
  • Neural Crest/metabolism*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Wnt Signaling Pathway
  • Cell Proliferation/physiology
  • Gene Expression Regulation, Developmental
  • Zebrafish
  • Lateral Line System/embryology*
  • Animals
  • Cell Differentiation/physiology
(all 12)
PubMed
32658373 Full text @ Dev. Dyn.
Abstract
Background The distribution of sensory organs is important for detecting environmental signals efficiently. The mechanosensory receptors of the lateral line system, neuromasts, are stereotypically distributed over the head and body surface of fish, although how neuromasts arise in these predetermined positions during development remains unclear.
Results We investigated development of the anterior lateral line (ALL) system in zebrafish head. The ALL neuromasts formed in the predetermined positions through proliferation and differentiation of 1) non-migratory lateral line primordia, 2) migratory primordia, 3) interneuromast cells connecting pre-existing neuromasts, and 4) budding primordia. We demonstrated that R-spondin2 (Rspo2), an activator of Wnt/β-catenin signaling, is required for the development of a particular set of neuromasts associated with hyomandibular cartilage. Further genetic analyses suggested that Rspo2, which emanates from the hyoid mesenchyme, acts on the adjacent neuromast progenitor cells to stimulate their proliferation through activating Wnt/β-catenin signaling.
Conclusion This study has revealed novel mechanisms for neuromast positioning through local tissue-tissue interactions, providing insights into the development and evolution of the vertebrate head. This article is protected by copyright. All rights reserved.
Genes / Markers
Figures
Figure Gallery (8 images)
Show all Figures
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
js201
    		Small Deletion
    nkhgn39dEtTransgenic Insertion
    nkhspGFFDMC13FEtTransgenic Insertion
      nkhspGFFDMC131AEtTransgenic Insertion
        nkuasgfp1aTgTransgenic Insertion
          nkuasrfp1aTgTransgenic Insertion
            nns6TgTransgenic Insertion
              nns8TgTransgenic Insertion
                vu234TgTransgenic Insertion
                  zf106TgTransgenic Insertion
                    1 - 10 of 11
                    Show
                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    Target Reagent Reagent Type
                    neurog1MO1-neurog1MRPHLNO
                    1 - 1 of 1
                    Show
                    Fish
                    No data available
                    Antibodies
                    No data available
                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    EGFPEFGEGFP
                    GAL4EFGGAL4
                    GAL4FFEFGGAL4FF
                    mRFPEFGmRFP
                    RFPEFGRFP
                    TomatoEFGTomato
                    1 - 6 of 6
                    Show
                    Mapping
                    No data available
                    Your Input Welcome
                    We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
                    Citation
                    Iwasaki, M., Yokoi, H., Suzuki, T., Kawakami, K., Wada, H. (2020) Development of the anterior lateral line system through local tissue-tissue interactions in the zebrafish head. Developmental Dynamics : an official publication of the American Association of Anatomists. 249(12):1440-1454.